Protein Info for PP_3591 in Pseudomonas putida KT2440

Annotation: Delta 1-piperideine-2-carboxylate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 99% identical to PY2CR_PSEPU: Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase (dpkA) from Pseudomonas putida

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3591)

MetaCyc: 100% identical to Delta1-piperideine-2-carboxylate reductase (Pseudomonas putida)
Pyrroline-2-carboxylate reductase. [EC: 1.5.1.1]

Predicted SEED Role

"Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1)" in subsystem Lysine degradation (EC 1.5.1.1, EC 1.5.1.21)

MetaCyc Pathways

L-lysine degradation V (9/9 steps found)
trans-3-hydroxy-L-proline degradation (1/2 steps found)
L-proline biosynthesis IV (1/2 steps found)
N-hydroxy-L-pipecolate biosynthesis (2/4 steps found)
L-lysine degradation II (L-pipecolate pathway) (5/9 steps found)
superpathway of L-lysine degradation (23/43 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.1 or 1.5.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GX6 at UniProt or InterPro

Protein Sequence (332 amino acids)

>PP_3591 Delta 1-piperideine-2-carboxylate reductase (Pseudomonas putida KT2440)
MRVPFTELQSLLQAIFQRHGCSEAVARVLAHNCASAQRDGAHSHGVFRMPGYVSTLASGW
VDGQATPQVSDVAAGYVRVDAAGGFAQPALAAARELLVAKARSAGIAVLAIHNSHHFAAL
WPDVEPFAEEGLVALSVVNSMTCVVPHGARKPLFGTNPIAFAAPCAEHDPIVFDMATSAM
AHGDVQIAARAGQQLPEGMGVDADGQPTTDPKAILEGGALLPFGGHKGSALSMMVELLAA
ALTGGHFSWEFDWSGHPGAKTPWTGQLIIVIDPGKAEGQRFAQRSRELVEHMQAVGLTRM
PGERRYREREVAEEEGVAVTEQELKGLKELLG