Protein Info for PP_3583 in Pseudomonas putida KT2440

Annotation: multidrug efflux transport system - membrane subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1035 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 336 to 353 (18 residues), see Phobius details amino acids 363 to 384 (22 residues), see Phobius details amino acids 390 to 410 (21 residues), see Phobius details amino acids 431 to 454 (24 residues), see Phobius details amino acids 464 to 487 (24 residues), see Phobius details amino acids 528 to 547 (20 residues), see Phobius details amino acids 857 to 875 (19 residues), see Phobius details amino acids 882 to 902 (21 residues), see Phobius details amino acids 908 to 932 (25 residues), see Phobius details amino acids 954 to 974 (21 residues), see Phobius details amino acids 986 to 1012 (27 residues), see Phobius details PF00873: ACR_tran" amino acids 7 to 1012 (1006 residues), 1071.2 bits, see alignment E=0 PF03176: MMPL" amino acids 254 to 504 (251 residues), 30.4 bits, see alignment E=3e-11 amino acids 789 to 1022 (234 residues), 26.9 bits, see alignment E=3.6e-10

Best Hits

KEGG orthology group: K07789, RND superfamily, multidrug transport protein MdtC (inferred from 70% identity to avn:Avin_28790)

Predicted SEED Role

"Acriflavin resistance plasma membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GY2 at UniProt or InterPro

Protein Sequence (1035 amino acids)

>PP_3583 multidrug efflux transport system - membrane subunit (Pseudomonas putida KT2440)
MNLSGPFIRRPVATMLLSLAIMLLGGVSFGLLPVAPLPQMDFPVIVVSANLSGASPEVMA
STVATPLERKLGSIAGVTTLTSSSNQGSTRVVIGFELGRDIDGAAREVQAAINATRNLLP
SGMRSMPTYKKINPSQAPIMVLSLTSDVLQKGQLYDLADTILSQSLAQVSGVGEVQIGGS
SLPAVRIAVEPQLLNQYNLSLDEVRTAVSNANQRRPMGFVEDAERNWQVRANDQLESAKD
YEPVVIRQQNGTILRLSDVATVTDGVENRYNSGFFNDQAAVLLVVNRQTGANIIETVDQI
KAQLPALQSLLPASVQLNVAMDRSPVIKATLKEAEHTLLIAVVLVILVVYLFLGSLRASL
IPSLAVPVSLVGTFAVMYVCGFSLNNLSLMALILATGLVVDDAIVVLENISRHIENGQPP
MKAAFLGAKEVGFTLLSMNVSLVAVFVSILFMGGIVRNLFQEFSITLAAAIIVSLVVSLT
LTPMLCARWLKPQQAEQTRLQRWSDTLHQRMVAAYDHSLGWALRHKRLTLLSLLATIGIN
IALYVVVPKTLMPQQDTGQLMGFIRGDDGLSFTVMQPKMEIYRRALLADPAVQSVAGFIG
GNSGTNNAFVLVRLKPISERKIDAQKVIERLRKELPKVPGGRLFLMADQDLQLGGGGRDQ
TSSQYLYTLQSGDLAALREWFPKVVAALRALPELTAIDARDGAGTQQVTLVVDRDQAKRL
GIDMDMVTAVLNNAYSQRQISTIYDSLNQYQVVLEINPKYAWDPSTLEQVQVITADGARV
PLSTIAHYENSLANDRVSHEGQFASEDIAFDVAEGYSPDQAMAALERAVAKLGLPEEVIA
KLGGTADAFAQTQQGQPFMILGALLLVYLVLGILYESYIHPLTILSTLPSAGVGALLALY
VTGGEFSLISLLGLFLLIGVVKKNAILMIDLALQLERHQGFSPEESIRRACLLRLRPILM
TTLAAILGALPLLLSQAEGAEMRQPLGLTIIGGLVFSQILTLYTTPVVYLYLDRLRHRFN
RWRGVRTDAALETPL