Protein Info for PP_3529 in Pseudomonas putida KT2440

Annotation: dimethyl-sulfide monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 TIGR03860: FMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family" amino acids 7 to 434 (428 residues), 553.8 bits, see alignment E=1.3e-170 PF00296: Bac_luciferase" amino acids 26 to 392 (367 residues), 146.7 bits, see alignment E=5.2e-47

Best Hits

Swiss-Prot: 52% identical to DMOA_HYPSL: Dimethyl-sulfide monooxygenase (dmoA) from Hyphomicrobium sulfonivorans

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3529)

MetaCyc: 52% identical to DmoA (Hyphomicrobium sulfonivorans)
RXN-9740 [EC: 1.14.13.131]

Predicted SEED Role

"Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.131

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88H36 at UniProt or InterPro

Protein Sequence (454 amino acids)

>PP_3529 dimethyl-sulfide monooxygenase (Pseudomonas putida KT2440)
MPRPIRFNAFSMNAASHQSPGLWRHPRNTSVAFNRLSYWTDLARLLERGLFDALFIADVL
GIYDVYQGSPQAALRGGVQVPVNDPLLLVPAMAGVTEHLGFGVTFSLTYEHPYPFARRMS
TLDHLSNGRVGWNIVTGYLDSAARNLGLARQRGHDQRYDLAEEYLQVLYKLWEKSWDDDA
VLLERESGRYIEPSRVHPINHVGEHFQVPGMHLCQPSPQRTPVLFQAGASARGQQFAARH
AECVFISGPTPTVLRRYADGIRQACEAAGRGRDEVLIYAQALLIVAPTREEAEARFNDYR
RYVDPDAALALLSGWTGIDFAGLDPDAPIEYVENDAGRAALAAFTAADPTRRWTVREAAE
FVGLGGRGPVLIGAASEVADQLETWLDHTGIDGFNLTYAVQPDDLANVVDLLVPELQRRG
RYPLSYTEGPLRHKLFGRGGQLPERHVGRQVSIQ