Protein Info for PP_3507 in Pseudomonas putida KT2440

Annotation: Aerobic cobaltochelatase subunit CobN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1253 TIGR02257: cobaltochelatase, CobN subunit" amino acids 1 to 1247 (1247 residues), 1728.5 bits, see alignment E=0 PF02514: CobN-Mg_chel" amino acids 138 to 1236 (1099 residues), 1330.9 bits, see alignment E=0

Best Hits

Predicted SEED Role

"CobN component of cobalt chelatase involved in B12 biosynthesis" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88H58 at UniProt or InterPro

Protein Sequence (1253 amino acids)

>PP_3507 Aerobic cobaltochelatase subunit CobN (Pseudomonas putida KT2440)
MHLLRTQPGGFVPDDSIADLGQTPAELVILCSGDSHLALLADTAEQLPEDFPSLRLANPM
QVQNHASVDLYVDQVLRHAKVILVSLHGGVGYWRYGVEQLVELAARGVQLILVPGDDRPD
PELTSLGNVRGEQAERLWHYLRQGGKANAINLFNCLASQWLGRDYAWDEPQPLPRTAVYH
PAKGSATLEDWYPHWHPEQPVAPLLFYRSHLQAANTAFIDVFCQRLQAAGLNPLPIAVAS
LKESACLEQVEAWLDEVGAEVLVNTTGFALSSPERPNLRPFRRDIPVLQAICAQDNQPGW
EASEQGLGARDLAMHIALPELDGRIITRPVSFKDMAWRSERSQSDVVCYRAHPERMDFVA
ELARRWVELARLPNAQKRVALVLANYPTRDGRIGNGVGLDTPAAALNILKALQAEGYPLA
ELPGSGTQLIHQLLGGVTNDLDHLDQRPCAQSLSLSDYQVAFERLPQANRQAVLERWGPP
EQDPMFRSGRLMVAGLRFGLTFVGIQPARGYQVDPSAVYHDPDLVPPHGYLAFHFWLRHA
FAADAVIHVGKHGNLEWLPGKGVGLSAQCWPDALLGPLPNIYPFIVNDPGEGAQAKRRTQ
AVIIDHLMPPLTRAETYGPLRHLEQLADEFYEAQLLDPRRARELQRDILELVKANHIDRE
LQLEGQLDDAALWLPRLDTYLCDLKESQIRDGLHVFGQSPEGRLRLDTLLALLRVERGDG
RGGNASLLRALAKALVPGWDPLDCDLGQPWQGIRPEQLQAMSNEPWRTCGDTRERLELLA
LQVIGQALEGTAQLPDLSEWQPVHAVVQALREAVAPSLDACGTAEMHGLLAALAGRFVPA
GPSGAPSRGRLDVLPTGRNFYTVDVRNLPTTTAWRLGFASASLILERHLQDHGDHLRQLG
LSVWGTATMRTGGDDIAQAMALMGVRPVWATGSQRVDDFEILPLSLLDRPRVDVTLRVSG
FFRDAFGNLIRLFDAAVQAVAALDEPEDLNPLAARVRSERAALQAQGVDAEQAARQAGWR
VFGAKPGAYGAGVQNAIDGRLWHSRDDLAEVYLNHGGYAYGGSDDGTPARTQFAQRLAKV
QAVLQNQDNHEHDLLDSNDYYQFQGGMLAASETLSGAVVASYHGDHSQADRPRIRTLKEE
LNRVIRARALNPKWIDGVKRHGYKGAFEMAATVDNLFAFDATTHLIDDHHYQGLADAYVL
DPATRDFMREHNPEALRDLTERLLEAQQRGLWQAPGDYREALEDQLLDGEEQA