Protein Info for PP_3497 in Pseudomonas putida KT2440

Annotation: putative peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 666 PF01136: Peptidase_U32" amino acids 79 to 304 (226 residues), 240.1 bits, see alignment E=2e-75 PF12392: DUF3656" amino acids 400 to 512 (113 residues), 65.7 bits, see alignment E=5.9e-22

Best Hits

KEGG orthology group: K08303, putative protease [EC: 3.4.-.-] (inferred from 100% identity to ppf:Pput_2274)

Predicted SEED Role

"Putative collagenase"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88H64 at UniProt or InterPro

Protein Sequence (666 amino acids)

>PP_3497 putative peptidase (Pseudomonas putida KT2440)
MSLPKNHLELLSPARDVAIAREAILHGADAIYIGGPSFGARHNACNEVSDIAELVEFAHR
YHARVFTTINTILHDNELEPARKLIHQLYDAGVDALIVQDLGVMELDIPPIELHASTQTD
IRTLERAKFLDQAGFSQLVLARELNLQQIRAIAAETDAAIEFFIHGALCVAFSGQCNISH
AQTGRSANRGDCSQACRLPYTLKDDQGRVVAFEKHLLSMKDNNQTANLADLVDAGVRSFK
IEGRYKDMGYVKNITAHYRKELDAILEDRPQYARASSGRTQHFFLPDPDKTFHRGSTDYF
VTDRKVDIGAFDSPTFTGLPVGVVEKVGKRDMQVVTDVPLTNGDGLNVLVKREVVGFRAN
IAEPRGEFEEDGQKRYRYRVEPNEMPEGLHKLRPNHPLSRNLDHNWQQALQRTSSERRVG
VEWHAVLREQRLMLTLSSEEGVSVQVALDGPFGVANKPQQALEQLHDLLGQLGTTLYHAD
RIELDAPQAYFIPNSQLKALRREAIEALTAARVQAHPRGGRKAETTPPPVYPESHLSFLA
NVYNQKARDFYHRHGVQLIDAAYEAHEEHGEVPVMITKHCLRFSFNLCPKQAKGVTGVRT
KVAPMQLIQGDEVLTLKFDCKPCEMHVIGKMKSHIIDLPTPGSAVAQVVGHISPEDLLKT
IPRGPH