Protein Info for PP_3467 in Pseudomonas putida KT2440

Annotation: Sigma-54 dependent transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 PF01590: GAF" amino acids 119 to 192 (74 residues), 40.9 bits, see alignment E=8.6e-14 PF00158: Sigma54_activat" amino acids 339 to 504 (166 residues), 216.1 bits, see alignment E=7.5e-68 PF14532: Sigma54_activ_2" amino acids 342 to 509 (168 residues), 74.3 bits, see alignment E=3.5e-24 PF07728: AAA_5" amino acids 360 to 468 (109 residues), 33.4 bits, see alignment E=1.3e-11 PF02954: HTH_8" amino acids 594 to 634 (41 residues), 48.6 bits, see alignment 1.6e-16

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3467)

Predicted SEED Role

"sigma-54 dependent transcriptional regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88H93 at UniProt or InterPro

Protein Sequence (636 amino acids)

>PP_3467 Sigma-54 dependent transcriptional regulator (Pseudomonas putida KT2440)
MARQLITNAHDPLHESREARLKLASEGELPLGMLRDEIDASWRRSLGHGLDCLQGEQVGL
GLEQGHDLRLLLERNRLLIDAVTPELDYLVARQGKAGIVILGDAQANVLAIEGQKHVLQR
EGLRDLHPGSCWSESLRGTNAIGTAVVEGRPTLINCGEHYLDRLSPFSCTSVPLRDPRGE
VIGVLDITREGVMAQPQDSLSTLMLAAGNIESRLFGLCHPDQLVLAFHSRPQYLNSAWHG
LLALSLDGEVLAANDGACQLLQVPRHELIGRRSTDLLGERSPAFIARLWQGGVSSVQTAK
GEFYFRALQLPRHGRVTPSTPSSKPALSKQSPALDALAGGDQRLARNLRMARQGLGNGLP
VLLLGETGTGKEVVARALHQASPRADKPFVAVNCAAIPEGLIESELFGYREGAFTGSRRG
GMVGRLMQAHGGTLFLDEIGDMPLALQARLLRVLQERRVAPLGAGDEQDIDVALICATHR
DLKRLVQEQHFREDLFYRVNGVSLRLPALRERDDLAAIIQGLLDKADARGVTLDPALTAL
LEGFDWPGNIRQLEMVVRTALAMREDGEQVLTLDHLTDCLLDELASSAAPSGSLKDSELE
QIRGALARHQGNVSAAAAALGISRATLYRKLKQLRG