Protein Info for PP_3463 in Pseudomonas putida KT2440
Annotation: phenylacetaldehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 53% identical to STYD_PSEFL: Phenylacetaldehyde dehydrogenase (styD) from Pseudomonas fluorescens
KEGG orthology group: K00146, phenylacetaldehyde dehydrogenase [EC: 1.2.1.39] (inferred from 99% identity to ppg:PputGB1_2474)MetaCyc: 98% identical to phenylacetaldehyde dehydrogenase monomer (Pseudomonas putida U)
Phenylacetaldehyde dehydrogenase. [EC: 1.2.1.39]
Predicted SEED Role
"Aldehyde dehydrogenase (EC 1.2.1.3)" in subsystem Entner-Doudoroff Pathway or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.1.3)
MetaCyc Pathways
- superpathway of phenylethylamine degradation (11/11 steps found)
- superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
- superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
- superpathway of glycol metabolism and degradation (6/7 steps found)
- ethanol degradation II (3/3 steps found)
- ethanol degradation IV (3/3 steps found)
- phenylethylamine degradation I (2/2 steps found)
- phenylethylamine degradation II (2/2 steps found)
- putrescine degradation V (2/2 steps found)
- mitochondrial NADPH production (yeast) (4/5 steps found)
- octane oxidation (4/5 steps found)
- superpathway of ornithine degradation (6/8 steps found)
- L-tryptophan degradation X (mammalian, via tryptamine) (3/4 steps found)
- phytol degradation (3/4 steps found)
- putrescine degradation III (3/4 steps found)
- L-phenylalanine degradation II (anaerobic) (2/3 steps found)
- ethanol degradation III (2/3 steps found)
- hypotaurine degradation (2/3 steps found)
- putrescine degradation IV (2/3 steps found)
- ethylene glycol degradation (1/2 steps found)
- phenylethanol degradation (1/2 steps found)
- putrescine degradation I (1/2 steps found)
- L-phenylalanine degradation IV (mammalian, via side chain) (6/9 steps found)
- D-arabinose degradation II (2/4 steps found)
- fatty acid α-oxidation I (plants) (2/4 steps found)
- serotonin degradation (4/7 steps found)
- histamine degradation (1/3 steps found)
- styrene degradation (1/3 steps found)
- 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
- noradrenaline and adrenaline degradation (8/13 steps found)
- Entner-Doudoroff pathway II (non-phosphorylative) (5/9 steps found)
- dopamine degradation (2/5 steps found)
- aromatic biogenic amine degradation (bacteria) (3/8 steps found)
- ceramide degradation by α-oxidation (2/7 steps found)
- sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
- alkane oxidation (1/6 steps found)
- limonene degradation IV (anaerobic) (1/7 steps found)
- ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (4/27 steps found)
- superpathway of pentose and pentitol degradation (10/42 steps found)
KEGG Metabolic Maps
- 1,2-Dichloroethane degradation
- 3-Chloroacrylic acid degradation
- Arginine and proline metabolism
- Ascorbate and aldarate metabolism
- Butanoate metabolism
- Fatty acid metabolism
- Glycerolipid metabolism
- Glycolysis / Gluconeogenesis
- Histidine metabolism
- Limonene and pinene degradation
- Lysine degradation
- Phenylalanine metabolism
- Propanoate metabolism
- Pyruvate metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.3
Use Curated BLAST to search for 1.2.1.3 or 1.2.1.39
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88H97 at UniProt or InterPro
Protein Sequence (497 amino acids)
>PP_3463 phenylacetaldehyde dehydrogenase (Pseudomonas putida KT2440) MLSELPILPATRAFLERKLKMRIGADWQDAASGRTLSFRNPATGEVLGEVPAAEAEDVDR AVRAARQAFDDSPWSRMRPRERQNLLWRLADLMERDARQLAELECLNNGKSAAVAQMMDV QLAIDFLRYMAGWATKIEGSTVEASMPLMPNDQFHGFVRREAVGVVGAIVAWNFPLLLAC WKLGPALATGCTVVLKPADETPLSVLKLAELVDEAGYPAGVFNVVTGTGLNAGAALSRHP GVDKLTFTGSTEVGKLIGKAAMDNMTRVTLELGGKSPTIVMPDANLQEAAAGAATAIFFN QGQVCCAGSRLYVHRKHFDNVVADIAGIANAMKLGNGLDPAVQMGPLISAKQQDRVTGYI ELGRELGATIACGGEGFGPGYFVKPTVIVDVDQRHRLVQEEIFGPVLVAMPFDDIDEVIG MANDNPYGLGASIWSNDLAAVHRMIPRIKSGSVWVNCHSALDPALPFGGYKMSGVGREMG SAAIEHYTELKSVLIKL