Protein Info for PP_3455 in Pseudomonas putida KT2440

Annotation: Multidrug efflux RND membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 40 to 376 (337 residues), 268.9 bits, see alignment E=2.6e-84 PF13533: Biotin_lipoyl_2" amino acids 65 to 112 (48 residues), 27.2 bits, see alignment 5.5e-10 PF00529: CusB_dom_1" amino acids 157 to 362 (206 residues), 33.8 bits, see alignment E=5.4e-12 PF13437: HlyD_3" amino acids 175 to 291 (117 residues), 38.3 bits, see alignment E=3.9e-13

Best Hits

Swiss-Prot: 48% identical to ACRA_ECO57: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli O157:H7

KEGG orthology group: K03585, membrane fusion protein (inferred from 100% identity to ppu:PP_3455)

MetaCyc: 48% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HA5 at UniProt or InterPro

Protein Sequence (391 amino acids)

>PP_3455 Multidrug efflux RND membrane fusion protein (Pseudomonas putida KT2440)
MPTTLSPCLRPLALMALLSLSLAGCSDSAEPDEQAPTAQVRVETLQLQPLAISSELSGRI
LAPRTAEVRARVAGVVLKRVYREGSDVKQGDVLFLIDPAPFKADHDSARATLAKAEATRY
QARLQEQRYRELVDDKAVSRQEYDNAKASFLQADAEVAEARAALERARLNLGYATVTAPI
SGRIGRAQVTEGALVGQNETTPLATIQQLDPIHADVTQSTRELNALRRALRAGELQQVGD
GQARATLIQDDGSAYPLPGKLLFSDISVDPSTNQITLRSEFPNPDLDLLPGSYVRVRLEQ
AVQPKGLSVPQRAILRDSAGVPKVLVVDQHARVSDRQVVLGSAQGDRWIVSEGLAAGERV
VVEGLQHVKAGDQVQVDNTPAAPPIAQHTGQ