Protein Info for PP_3452 in Pseudomonas putida KT2440

Annotation: GGDEF domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 363 transmembrane" amino acids 33 to 55 (23 residues), see Phobius details amino acids 61 to 80 (20 residues), see Phobius details amino acids 88 to 106 (19 residues), see Phobius details amino acids 112 to 130 (19 residues), see Phobius details amino acids 137 to 154 (18 residues), see Phobius details amino acids 166 to 184 (19 residues), see Phobius details TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 202 to 352 (151 residues), 118.1 bits, see alignment E=1.7e-38 PF00990: GGDEF" amino acids 204 to 351 (148 residues), 125.7 bits, see alignment E=7.8e-41

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3452)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HA8 at UniProt or InterPro

Protein Sequence (363 amino acids)

>PP_3452 GGDEF domain protein (Pseudomonas putida KT2440)
MFKTIEAHIRQQVAPAALRAEFRQHEFESMRSFCLLVFSISVLIWLVFDLIVSFLGDQGF
TWLSFLFIALLGSLIVVLHFTRRSHHFDVLNLLFITVITLGMRLVIEGIPIALRPVWLVL
GVSTVLYAMSVLPVRRWSFFCAMVITWVMLNPFYHTRIEPDALEGAMLISYAVFLSGLVS
YSYLQIRKAKLHNFYLSKVLLEQAYVDALTDIPNRRSFMAQADRQLRMATSGQYLAMIDI
DNFKRVNDRFGHDVGDEVLKRVALHIKTSMADQAFARLGGEEFAIFFEGLDQQGAEQRVA
ALCQRVREDAGEHPVTISIGLAKVDRGESLTTALVRADQALYEAKHTGKDRFVSWSSELA
EQD