Protein Info for PP_3425 in Pseudomonas putida KT2440

Annotation: Efflux transporter RND family, MFP subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 38 to 368 (331 residues), 264.4 bits, see alignment E=5.9e-83 PF13533: Biotin_lipoyl_2" amino acids 62 to 111 (50 residues), 51.9 bits, see alignment 7.7e-18 PF16576: HlyD_D23" amino acids 62 to 287 (226 residues), 53.5 bits, see alignment E=3.1e-18

Best Hits

Swiss-Prot: 34% identical to BEPF_BRUSU: Efflux pump periplasmic linker BepF (bepF) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3425)

Predicted SEED Role

"Multidrug efflux membrane fusion protein MexE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HD5 at UniProt or InterPro

Protein Sequence (413 amino acids)

>PP_3425 Efflux transporter RND family, MFP subunit (Pseudomonas putida KT2440)
MEQSLKPLRFPLAALAVVVISACGRTPDAVQAPAAPKVSVAKVIEQPITEWDEFTGRLEA
PETVEVRPRVSGQIDQVAFTEGAQVKKGDLLFQIDPRPFQAEVRRLEAQLQQAKATAIRS
ANEARRGERLRDSNAISAELAESRSSAAAEARAGVDAIQAQLDLARLNLSFTRVTAPISG
RVSRAEFTAGNIVTADVTPLTSVVSTDKVYAYFDADERVYLKYTQLAREGQRSQSTPVYL
GLTNESGNPHLGQMNFVDNQVNPRTGTIRGRAVFDNSNGQFTPGLYARLKLVGSAQYDAM
LINDEAVGTDLGKKFVLVMDKDNKATYRAVELGPKLEGLRIVRSGLGKDDRIVVKGLQRV
RPGSPVTPEETQMASEQTLAALAQQRQALEASNPAPKVAGNNVKVASAQAPRG