Protein Info for PP_3417 in Pseudomonas putida KT2440

Annotation: D-gluconate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 transmembrane" amino acids 9 to 27 (19 residues), see Phobius details amino acids 33 to 52 (20 residues), see Phobius details amino acids 62 to 81 (20 residues), see Phobius details amino acids 108 to 136 (29 residues), see Phobius details amino acids 144 to 162 (19 residues), see Phobius details amino acids 182 to 206 (25 residues), see Phobius details amino acids 230 to 249 (20 residues), see Phobius details amino acids 269 to 289 (21 residues), see Phobius details amino acids 307 to 326 (20 residues), see Phobius details amino acids 348 to 380 (33 residues), see Phobius details amino acids 390 to 413 (24 residues), see Phobius details amino acids 428 to 449 (22 residues), see Phobius details PF02447: GntP_permease" amino acids 10 to 449 (440 residues), 604.1 bits, see alignment E=1.5e-185 TIGR00791: transporter, gluconate:H+ symporter (GntP) family" amino acids 15 to 450 (436 residues), 539.7 bits, see alignment E=3.1e-166 PF03600: CitMHS" amino acids 22 to 394 (373 residues), 51.3 bits, see alignment E=1e-17

Best Hits

Swiss-Prot: 81% identical to GNUT_PSEAE: Gluconate permease (gnuT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03299, gluconate:H+ symporter, GntP family (inferred from 100% identity to ppf:Pput_2345)

MetaCyc: 37% identical to fructuronate transporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-81; TRANS-RXN0-209

Predicted SEED Role

"Gluconate permease" in subsystem D-gluconate and ketogluconates metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HE3 at UniProt or InterPro

Protein Sequence (450 amino acids)

>PP_3417 D-gluconate transporter (Pseudomonas putida KT2440)
MFGLATDTFLLLDALVTIVGLILLITHFKVHPFVALTLAAGFLGLTSGMPVAKVMKSFQD
GFGGVLGFVGIVLALGTMLGKLMADSGGADQIAQTLIRAFGKKNVHWAMMFAAFLVGIPL
FFEIGFVLLIPLVFIVARRSGVSLVKIGIPLLAGLSVVHGLVPPHPGPLLAIGIFHADIG
KTIFYGLIVALPTAIIAGPLFGNFISRYIPGNPSQELMDQIAKDSDQGNLPSFSITLVTV
LLPVALMLLKTFADVVLPAEHIVRQWMDLIGHPITALLAALLLAFYTFGSARGFNRQQIM
KMLDQSLAPTAAIVLIVGAGGGFKQMLVDTGVGNVIGQMAVQAEISPIMLAWLVAAVIRI
ATGSATVATITGAGIVAPVIDLVPGVNRELLVLATGAGSLILSHVNDAGFWLVKQYFNMT
VAETFKTWSMMETILSIVGIIFIMLLSLVV