Protein Info for PP_3414 in Pseudomonas putida KT2440

Annotation: Methyl-accepting chemotaxis transducer/sensory box protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 TIGR00229: PAS domain S-box protein" amino acids 32 to 142 (111 residues), 70.7 bits, see alignment E=6e-24 amino acids 144 to 265 (122 residues), 57.4 bits, see alignment E=8e-20 PF08448: PAS_4" amino acids 36 to 139 (104 residues), 54.3 bits, see alignment E=4.5e-18 amino acids 155 to 260 (106 residues), 42.7 bits, see alignment E=1.8e-14 PF00989: PAS" amino acids 39 to 135 (97 residues), 31.3 bits, see alignment E=5.2e-11 PF13426: PAS_9" amino acids 40 to 137 (98 residues), 49.6 bits, see alignment E=1.2e-16 amino acids 159 to 258 (100 residues), 46.8 bits, see alignment E=9.4e-16 PF08447: PAS_3" amino acids 46 to 132 (87 residues), 55 bits, see alignment E=2.5e-18 amino acids 168 to 253 (86 residues), 52.6 bits, see alignment E=1.4e-17 PF00015: MCPsignal" amino acids 277 to 435 (159 residues), 124.6 bits, see alignment E=1.2e-39

Best Hits

Swiss-Prot: 50% identical to BDLA_PSEAE: Biofilm dispersion protein BdlA (bdlA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03406, methyl-accepting chemotaxis protein (inferred from 100% identity to ppu:PP_3414)

Predicted SEED Role

"Methyl-accepting chemotaxis protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HE6 at UniProt or InterPro

Protein Sequence (439 amino acids)

>PP_3414 Methyl-accepting chemotaxis transducer/sensory box protein (Pseudomonas putida KT2440)
MFDFHRKSDLAEIQRTRQALADAQAKLAAIGRSMAMIEFAPDGTILDANERFCQAMGYSA
DELRGKHHRLFCEPDYAKSAEYQQLWRELGQGKAISGTFERIDKAGREVWLEASYMPVLD
EHRQVTSVIKVASDITQRVVQEHESESLLKAISRSMAVIEFTPQGRVIKANQNFLDTMGY
RLDEVVGRHHGLFCLAHERESAQYREFWASLNRGEYHSHRFERVNKQGQTVFLEASYNPI
FDSKGRLCKVVKFASDITHQVCTQQSAAHAAHASSVQTDACARKGTEVVQQAVQVIEQIS
QELNDAARSIDAVNRQSDVIGQIVLTIRGIADQTNLLALNAAIEAARAGEHGRGFAVVAD
EVRNLAARTSKATLEIVDVVRQNHDLSLLAVASMQSSLNRTGHGVALANEAGTVIMEIQQ
GSRHVVDAISQITSTLQLH