Protein Info for PP_3413 in Pseudomonas putida KT2440

Annotation: Sensor histidine kinase/response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1093 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 535 to 556 (22 residues), see Phobius details PF00497: SBP_bac_3" amino acids 62 to 272 (211 residues), 49 bits, see alignment E=1.6e-16 amino acids 302 to 518 (217 residues), 61 bits, see alignment E=3.1e-20 PF00512: HisKA" amino acids 599 to 664 (66 residues), 60.6 bits, see alignment 3.6e-20 PF02518: HATPase_c" amino acids 711 to 824 (114 residues), 82.3 bits, see alignment E=1.1e-26 PF00072: Response_reg" amino acids 848 to 961 (114 residues), 85.1 bits, see alignment E=1.2e-27 PF01627: Hpt" amino acids 998 to 1080 (83 residues), 33.2 bits, see alignment 1.5e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3413)

Predicted SEED Role

"Virulence sensor protein bvgS precursor (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HE7 at UniProt or InterPro

Protein Sequence (1093 amino acids)

>PP_3413 Sensor histidine kinase/response regulator (Pseudomonas putida KT2440)
MKQLLASVLLAIGLANSTELQASSEPRHLLARSISAAAPLTLSGDDRHWLKQRERLVMGS
ARPDYPPFEINVSQHDYEGLSADYAGIIGEQLDIPIEVRRFDSRHEAITALRDGRIDLLG
SSNAFEAADAQLSLSTPYADDLPVIVTREGRSLKSTPELAGLRLAMVDHYLPAVSVRTLY
PKAQLSLYRSTLAGLAAVALGEADAYLGDAISTDYMIGKSFQGTLKIDHFSQLAPEAFAF
AIASDNPRLHRLVDKALSRISESERLNILRRWSSGNTSLLLQRHLTALSEEEESWITAHP
TISVLVNTSLAPLTFNDAQHRPSGITLDLLKQISLRTGLQFTPVESASSQAMIDRLVRGE
AQMIGALGYGADRLKQLRYTRPYLVSPRVLVTRNDGESPAQAKALDGKRIALVRGSPQQA
VLQQRYPQAEIVEVDNPLGQMEAVANGVADAALSSHINAAYYISHVFKDRLRIANMLDDA
PAIAAFAVAADQPQLQAILDKALLSIPPEELDQLINRWRTSTLVSDSPWRDYRTLALQVL
VLSALLLAGVVFWNSYLRKLINQRTEAQHALQAQLALSRGLLEQLRQAKDDAEQASQTKS
TFLATMSHEIRTPMNAVIGLLELALEDSRSGRSDAQTLQTAHDSAIGLLELIGDILDISR
IESGHISLQPVPTNLVELVRATLRVFEGNARAKGLHLHGELPAEPVWVLADPLRLKQILS
NLISNAIKFTDRGEVQACLLLPKVAGHGILAIELNVRDTGIGISPADQARLFSAFVQVDG
PRARQGAGLGLVISRTLAELMGGALSLQSVEGVGTRVQVSLRLPVCEAPAQADPQLTTHE
SNSGPLNILVVDDYPANLLLLERQLQTLGHHVTLAENGEIALARWQEARFDLVITDCSMP
VMDGHELTRRIRSLEGERGLPAGRILGVTANAQAEERTRCLASGMDECLFKPIGLRTLKT
HLPQVRPQQPSPEPFHSGFDLAELRHLTQDDEQLTRHLLEQLSISVGEDLAALRALSTDA
PDETVRALAHRIKGGAKMIRVRTVVKDCEAIEHAHAKGLPTVEQRAQLQVSLQALLDEMR
EALNAIAASRLSG