Protein Info for PP_3385 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 282 transmembrane" amino acids 243 to 261 (19 residues), see Phobius details TIGR03349: type IV/VI secretion system protein, DotU family" amino acids 59 to 267 (209 residues), 221.6 bits, see alignment E=4.8e-70 PF09850: DotU" amino acids 61 to 263 (203 residues), 209.3 bits, see alignment E=2.6e-66

Best Hits

KEGG orthology group: K11892, type VI secretion system protein ImpK (inferred from 100% identity to ppu:PP_3385)

Predicted SEED Role

"Outer membrane protein ImpK/VasF, OmpA/MotB domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HH3 at UniProt or InterPro

Protein Sequence (282 amino acids)

>PP_3385 conserved protein of unknown function (Pseudomonas putida KT2440)
MPKESDRTGKPVPAHQKPILLEDTNETPRPPGGAGPEYATDLTSYPADPDFQLRGGYDNL
MLDAASQLFGLVIRLRTLDDLPNIQFVHKTVQTQIGTIREEMRQHGYCAVHQEVYSYALC
LHLDEAVMTRPWGQNSCWSHEPLLSIFHDETQGGEKIFVLLERIMQSPKEFQDVLEFLYF
CFCLGLQGKYALDPKGDEILRALISRMHKVIRQLRGPTPETVSDPYSNVVHCPHRMRRWQ
WPWWTPLVISVIAMVCAYSYYSYRLDLLTAEVVESLNAILQQ