Protein Info for PP_3354 in Pseudomonas putida KT2440

Annotation: putative Acyl-CoA dehydrogenase, ferrulic acid biotransformation protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 PF12418: AcylCoA_DH_N" amino acids 3 to 34 (32 residues), 50.6 bits, see alignment (E = 3.8e-17) PF02771: Acyl-CoA_dh_N" amino acids 40 to 156 (117 residues), 28.7 bits, see alignment E=3.7e-10 PF02770: Acyl-CoA_dh_M" amino acids 162 to 268 (107 residues), 45.4 bits, see alignment E=1.9e-15 PF00441: Acyl-CoA_dh_1" amino acids 280 to 448 (169 residues), 69.7 bits, see alignment E=8e-23 PF12806: Acyl-CoA_dh_C" amino acids 467 to 575 (109 residues), 40.9 bits, see alignment E=5.3e-14

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3354)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HK2 at UniProt or InterPro

Protein Sequence (584 amino acids)

>PP_3354 putative Acyl-CoA dehydrogenase, ferrulic acid biotransformation protein (Pseudomonas putida KT2440)
MTWKAPLRDMQFVLQHWLRADQAWAQLPAYADVDLDLAAQVLEEAARFCEQVLAPLNSSG
DRQGCQMEGGEVRTPDGFPAAYRAYVDGGWPSLACAPALGGQGLPLVLEAALQEMLFGSN
HGWAMYTGIAHGAYLCLKAHASAELQARYLPGIVSGATLPTMCLTEPQAGSDLSLLRCRA
VPLGDGGDGGYAVSGNKLFISGGEHDLTRDILHLVLARLPDAPPGNRGLSLLLVPKWLED
GQRNTLYCDGLEHKLGIRGSATCALRLEGARGWLIGQPHGGLAAMFVMMNSARLHVGLQG
LGHAEAAWQQARDYALERRQMSAPGRAKGGAQGADPIHLHPAMRRVLLELRVRTEGMRAL
AYWTAQWLDIADAAVEPAQRAKAATLAALLTPIVKAAFTEQGFSLASKALQVLGGYGYTQ
EFAIEQTLRDSRIAMIYEGTNEIQANDLMLRKVLGDGGEGFALLLSELRTEAAADAGAAA
AALGSACDTLEAALAKVLSMTAGDPEFPYRAAGDFLQLCATVLQAFAWARSARCIQALPV
GAAQRREKQESVGFFLGYLLPDLDRQVAAIDAAATPLPFIAESF