Protein Info for PP_3315 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 156 transmembrane" amino acids 33 to 58 (26 residues), see Phobius details amino acids 70 to 87 (18 residues), see Phobius details amino acids 99 to 117 (19 residues), see Phobius details amino acids 128 to 148 (21 residues), see Phobius details PF06146: PsiE" amino acids 30 to 152 (123 residues), 109.5 bits, see alignment E=6.3e-36

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3315)

Predicted SEED Role

"FIG00959020: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HN8 at UniProt or InterPro

Protein Sequence (156 amino acids)

>PP_3315 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MKDSDEPETTRMRLRREWAVLTFYERFEQIVAHILSLVIAVVIVVSLVQLIQIVFSLLII
DAFNPLDHRIFQNVFGMVMTLLIAMEFKHSIVRVVLRHGSIIQVKTVLLIGLIALSRKIV
ILDPDVSPAKVAALAGATLALGLTYWLLRERDDRVA