Protein Info for PP_3304 in Pseudomonas putida KT2440

Annotation: Multidrug resistance transporter, Bcr/CflA family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 412 transmembrane" amino acids 26 to 47 (22 residues), see Phobius details amino acids 62 to 80 (19 residues), see Phobius details amino acids 92 to 111 (20 residues), see Phobius details amino acids 117 to 138 (22 residues), see Phobius details amino acids 150 to 170 (21 residues), see Phobius details amino acids 178 to 198 (21 residues), see Phobius details amino acids 229 to 254 (26 residues), see Phobius details amino acids 266 to 284 (19 residues), see Phobius details amino acids 295 to 314 (20 residues), see Phobius details amino acids 320 to 341 (22 residues), see Phobius details amino acids 353 to 375 (23 residues), see Phobius details amino acids 382 to 405 (24 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 24 to 404 (381 residues), 215.7 bits, see alignment E=6.9e-68 PF07690: MFS_1" amino acids 31 to 370 (340 residues), 163.7 bits, see alignment E=5.7e-52 PF00083: Sugar_tr" amino acids 56 to 195 (140 residues), 38.8 bits, see alignment E=5.7e-14

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3304)

Predicted SEED Role

"multidrug resistance transporter, Bcr/CflA family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HP9 at UniProt or InterPro

Protein Sequence (412 amino acids)

>PP_3304 Multidrug resistance transporter, Bcr/CflA family (Pseudomonas putida KT2440)
MNSRLETHANEAEGDAQRANVLTAKVILLLAALAAISNLSTNIILPAFPEMARQLNVSSQ
ELGLTLSSFFITFAFAQLLVGPLADRYGRKRLVVGGLMIFVVGTFWAANAATLDMLILGR
VIQAIGVCAAAVLARAIARDLYEGENLARALSLTMIAAATAPGFSPLIGSMLNTTLGWRA
LFIAVGMSAILIALFYLRGIGETLPAHRRVTQSVPAILIAYGKLASNRLFILPALATSLL
MSGLFASFAAAPSILMEGMGLSSLQVGLYFAATVFVVFAAGLAAPRLAHRWGSRAITLSG
LATACTAGALLLVGPSNPSLGWYSLSMVLFLWGMGIANPLGTALTMTPFGKEAGLASALL
GFLTMAIGAVSTWMVSTPKFPTIAVLGATQTSVCFLAMVLFAWYVKRKAIVR