Protein Info for PP_3272 in Pseudomonas putida KT2440

Annotation: acetate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 46 to 68 (23 residues), see Phobius details amino acids 74 to 92 (19 residues), see Phobius details amino acids 116 to 138 (23 residues), see Phobius details amino acids 146 to 170 (25 residues), see Phobius details amino acids 179 to 200 (22 residues), see Phobius details amino acids 234 to 256 (23 residues), see Phobius details amino acids 268 to 293 (26 residues), see Phobius details amino acids 326 to 354 (29 residues), see Phobius details amino acids 375 to 394 (20 residues), see Phobius details amino acids 400 to 422 (23 residues), see Phobius details amino acids 432 to 455 (24 residues), see Phobius details amino acids 467 to 488 (22 residues), see Phobius details TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 33 to 439 (407 residues), 192.4 bits, see alignment E=7.3e-61 PF00474: SSF" amino acids 33 to 439 (407 residues), 383.6 bits, see alignment E=5.8e-119

Best Hits

Swiss-Prot: 69% identical to ACTP_SALG2: Cation/acetate symporter ActP (actP) from Salmonella gallinarum (strain 287/91 / NCTC 13346)

KEGG orthology group: K14393, cation/acetate symporter (inferred from 100% identity to ppf:Pput_2487)

MetaCyc: 68% identical to acetate/glycolate:cation symporter (Escherichia coli K-12 substr. MG1655)
RXN0-1981; RXN0-5111; TRANS-RXN0-576

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HT1 at UniProt or InterPro

Protein Sequence (520 amino acids)

>PP_3272 acetate permease (Pseudomonas putida KT2440)
MNWTAISMFMVFVCFTLLVTRWAALRTRSASDFYTAGGGLTGMQNGLAIAGDMISAASFL
GISAMMFMNGYDGLLYALGVLAGWPIILFLIAERLRNLGKYTFADVVSYRLAQTPVRLTS
AFGTLVVALMYLVAQMVGAGKLIELLFGISYLYAVMLVGVLMVSYVTFGGMLATTWVQII
KAVMLLSGTSFMAFMVLKHFGFSTEAMFASAVAVHAKGQAIMAPGGLLSNPVDAISLGLG
MMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYLLLIVVGFGAIVMVGTEPSYR
DATGAIIGGGNMVAVHLAQAVGGNLFLGFISAVAFATILAVVAGLALSGASAVSHDLYAC
VMRKGKATEQEEMRVSRIATLLIGLLAVILGLMFESQNIAFLSGLVLAVAASVNFPVLLL
SMFWKGLTTRGAVCGSMAGLVSAVLLVVLGPAVWVNVLHNETALFPYSNPALFSMSLAFL
SAWVFSVTDTSERAVEERGRYLGQFIRSMTGIGAAGASKH