Protein Info for PP_3232 in Pseudomonas putida KT2440

Annotation: Acetyltransferase, GNAT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 189 PF13302: Acetyltransf_3" amino acids 33 to 171 (139 residues), 30 bits, see alignment E=8.1e-11 PF00583: Acetyltransf_1" amino acids 83 to 170 (88 residues), 36.8 bits, see alignment E=4.3e-13

Best Hits

Swiss-Prot: 58% identical to ATSE4_PSEAE: Acetyltransferase PA5475 (PA5475) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3232)

Predicted SEED Role

"Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HX1 at UniProt or InterPro

Protein Sequence (189 amino acids)

>PP_3232 Acetyltransferase, GNAT family (Pseudomonas putida KT2440)
MAPAETSRVHTTPSFPKPSGEHWIEALENGVHVLIRPLQAQDREREKAFIERLSPESRHF
RFLCQLREPGEAMLDQLMKVDQDRQAAYVALAHVDGELQEVGISRYATASGKDECECAVT
VMDSWQHHGLGRLLLKHLIDHARAKGLRQMYSVDAAANQAMRELAKAAGFTTAADPDDPS
QVIHRLSLI