Protein Info for PP_3225 in Pseudomonas putida KT2440
Annotation: D-isomer specific 2-hydroxyacid dehydrogenase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 100% identity to ppu:PP_3225)Predicted SEED Role
"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)
MetaCyc Pathways
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
- L-serine biosynthesis I (3/3 steps found)
- L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.95
Use Curated BLAST to search for 1.1.1.95
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88HX8 at UniProt or InterPro
Protein Sequence (314 amino acids)
>PP_3225 D-isomer specific 2-hydroxyacid dehydrogenase family protein (Pseudomonas putida KT2440) MNHSIIASQLDDQANQHLREHLPGHEVIDIAPDQLAVEAPADVLILRPINVRGRRVGTPP PGWPWGLKWVQLVSSGIDFYPDWVFQGATVTSGKGANAEQVAEFALALVFAAAKQLPGLW VKDADWRLTPLAPLRGRTLGILGFGSIGQSLARKAVALGLRVLALSRPGQGIADIPGVEP VADLRQLFAGSDHLVLAAPLTAATRGLIDRQVLAYARPGLHLINIARGGLLDQQALLDAL DSGLIGRASLDVTEPEPLPAGHPLYHHPRVFLSPHTSAISEDGYPAFLDAFIANFHRYRE QAPLANLVDTARGY