Protein Info for PP_3209 in Pseudomonas putida KT2440

Annotation: Isoxanthopterin deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 PF01979: Amidohydro_1" amino acids 59 to 425 (367 residues), 154 bits, see alignment E=6.6e-49 PF07969: Amidohydro_3" amino acids 112 to 409 (298 residues), 33.6 bits, see alignment E=3.3e-12

Best Hits

Swiss-Prot: 62% identical to IXPDE_UNKP: Isoxanthopterin deaminase from Unknown prokaryotic organism

KEGG orthology group: K03382, hydroxyatrazine ethylaminohydrolase [EC: 3.5.99.3] (inferred from 100% identity to ppu:PP_3209)

Predicted SEED Role

"BOX elements"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.99.3

Use Curated BLAST to search for 3.5.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HZ3 at UniProt or InterPro

Protein Sequence (476 amino acids)

>PP_3209 Isoxanthopterin deaminase (Pseudomonas putida KT2440)
MNHTNSWLIRNASAILTGLRGGAARHVGPDIRITQGRIAALGSLQPLPGEQVLDATDCVV
YPGWVNTHHHLFQSLLKGIPAGIDQSLGAWLGAVPYRYRGLFDEHSFRLAARVGLVELAR
SGCSTVADHHYLYLPGMAFDSAQILFEEAERLGLRFILCRGGATQVRQAEGPATAGPETL
TQYLDDVQRLVQVYHDPADDAWRRVVMAPTSPPYSMPPEHLRETARAARRLGIRLHSHLS
ETVEYQNEVHDRHGLSPVVFCAEQEWLGPDVWFAHLVKLSAEEIQLLGATGTGIAHCPQS
NGRLGSGIADIVAMEAAGMSVSIGVDGAASNEACDMISETHAAWLMQRAKAGERAMARHA
GGDLEGGAHVASVEDVVRWGTAGGAQVLGLDAIGTLQVGMAADLAIYRLDQPRYFGLHDL
GIAPVVGGGRPTLRALLVGGRLSVENDAIPGLDLAELGSQARQAVLRLQQAAGVRA