Protein Info for PP_3167 in Pseudomonas putida KT2440

Annotation: benzoate transport protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 transmembrane" amino acids 33 to 59 (27 residues), see Phobius details amino acids 71 to 89 (19 residues), see Phobius details amino acids 96 to 114 (19 residues), see Phobius details amino acids 120 to 139 (20 residues), see Phobius details amino acids 146 to 164 (19 residues), see Phobius details amino acids 170 to 187 (18 residues), see Phobius details amino acids 194 to 213 (20 residues), see Phobius details amino acids 233 to 254 (22 residues), see Phobius details amino acids 272 to 294 (23 residues), see Phobius details amino acids 314 to 332 (19 residues), see Phobius details amino acids 338 to 358 (21 residues), see Phobius details amino acids 378 to 407 (30 residues), see Phobius details TIGR00843: benzoate transporter" amino acids 27 to 407 (381 residues), 405.2 bits, see alignment E=1.4e-125 PF03594: BenE" amino acids 31 to 406 (376 residues), 549.6 bits, see alignment E=1.8e-169

Best Hits

Swiss-Prot: 46% identical to BENE_ACIAD: Benzoate membrane transport protein (benE) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K05782, benzoate membrane transport protein (inferred from 100% identity to ppu:PP_3167)

Predicted SEED Role

"Benzoate transport protein" in subsystem Benzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88I34 at UniProt or InterPro

Protein Sequence (423 amino acids)

>PP_3167 benzoate transport protein (Pseudomonas putida KT2440)
MPAKQPTPKQEPATKAGRCLARSFMKTLIKDCSVSAIVAGSIATTISYAGPLVIIFHAAE
AAGLSHQQLSSWVWAVSMGSALLGALLSLRYRVPVVIAWSIPGSALLVTALPQLGLEQAV
GAYLVANLVLLLIGISGAFDRIIARLPGSIAAGMQAGILFSFGIEVFRALPVQPLLVLAM
FVTYVLMRRVQPRYAVAAVLGVGMLVTVASGTLRSEALVLELASPQWITPQFSLSAVFSL
ALPMVLVALTGQFMPGMAVLRNAGYPTPASPLISASALIGVLLAPFGCHGLNLAAVTASL
CTGHEAHENPQRRYVAAVAGSVLYLLLGIAGATLMSLFAAFPAVLIAALAGLALYGAISE
ALVRSLAEPAERDAGLFTFLVTASGVAFLGLSAAFWGLLFGLLAHALLRLRQPRAREVLR
RTP