Protein Info for PP_3119 in Pseudomonas putida KT2440

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1033 TIGR00594: DNA polymerase III, alpha subunit" amino acids 14 to 978 (965 residues), 586.7 bits, see alignment E=5.1e-180 PF02811: PHP" amino acids 15 to 132 (118 residues), 72.1 bits, see alignment E=1.8e-23 PF07733: DNA_pol3_alpha" amino acids 277 to 532 (256 residues), 303.1 bits, see alignment E=5e-94 PF17657: DNA_pol3_finger" amino acids 535 to 701 (167 residues), 201.6 bits, see alignment E=1.4e-63 PF14579: HHH_6" amino acids 774 to 864 (91 residues), 73.2 bits, see alignment E=4.1e-24 PF01336: tRNA_anti-codon" amino acids 944 to 1017 (74 residues), 37.3 bits, see alignment 5.4e-13

Best Hits

Swiss-Prot: 100% identical to DNAE2_PSEPK: Error-prone DNA polymerase (dnaE2) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 100% identity to ppu:PP_3119)

Predicted SEED Role

"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88I82 at UniProt or InterPro

Protein Sequence (1033 amino acids)

>PP_3119 error-prone DNA polymerase (Pseudomonas putida KT2440)
MAAGLVRMNTPGYAELHCLSNFSFQRGASSADELFRRAREQGYQALAITDECTLAGIVRA
WQAAKVHQLRLIVGSEVQLCDGPKLVLLVENLTGYQNLCALITRARRRAEKGAYQLFRDD
LLLHHQGLLALWVAADSGDTATGAWLRSVFAERLWLAVHLHRGSDDAVRLQRLRALAADV
GIRAVACGDVHMHVRGRRALQDCMTAIRQHCQVSEAGRFLFANGERHLRSQAQLAELYPL
DLLAETLVIARRCQFDLSELNYQYPRELVPEGHTPASWLRELCEQGMPLRWPDGPSGKVR
DVLAKELGLIEELGYESYFLTVHDIVAFARSQRILCQGRGSAANSVVCFVLGITELDPMK
HHLLFERFLSRERNEPPDIDVDFEHDRREEVIQYVFRRYGRHRAALTAVVNTYHAAGAVR
DVARALGLPADQVDALAKCCGRWSDRIPDDQRLAEAGFEAGSPSLRRVLVLAGELIGFPR
HLSQHPGGFVISQQPLDQLVPVENAAMPERTVIQWDKDDLDMVGLLKVDVLALGMLSALR
RCFDQLQHHRGRHLTLATIPSEDPATYAMISRAETMGVFQIESRAQMAMLPRLRPQKFYD
LVIEVAIVRPGPIQGDMVHPYLRRRLKQEPVTYPSPQLKEVFERTLGVPLFQEQVMELAM
VAADYTPGEADQLRRSMAAWKRHGGLEPHRERLVQGMLRNGYTLAFAERIFEQIKGFGSY
GFPESHAASFALLCYASSWLKCHEPAIFTCALVNSWPMGFYSPDQLLQEARRQGIEVRPV
DVCHSDWDCTLEPDAEGTLAIRMGLRLVRGLAEADAKRVQQARSQRPWRNVEDLCLRAGL
DARARARLADGGALRALASDRHQARWQVAAVQPQLPLFADVQALPEEPVQLPVPTVGEDL
MADYQTLGTTLGPHPLALLRARLRALGCRSSSELQGVEHGDNIAVAGLVVGRQRPQTASG
VTFVTLEDEHGMVNVVVWRALAERQRRALVGSQLLKVSGRLEQENGVRHLIARRLEDVSP
LLQGLDVRSRDFH