Protein Info for PP_3087 in Pseudomonas putida KT2440

Annotation: putative excinuclease ABC subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 838 PF17755: UvrA_DNA-bind" amino acids 163 to 275 (113 residues), 44.7 bits, see alignment E=3.7e-15

Best Hits

KEGG orthology group: K03701, excinuclease ABC subunit A (inferred from 65% identity to gag:Glaag_1820)

Predicted SEED Role

"Excinuclease ABC subunit A paralog of unknown function" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IB2 at UniProt or InterPro

Protein Sequence (838 amino acids)

>PP_3087 putative excinuclease ABC subunit A (Pseudomonas putida KT2440)
MPPSPTTPSGFIRVRGAREHNLKNIDVDIPRDALVVFTGVSGSGKSSLAFSTLYAEAQRR
YFESVAPYARRLIDQVGVPDVDAIDGLPPAVALQQQRGTPSARSSVGSVTTLSSSIRMLY
SRAGHYPPGQAMLYAEDFSPNTPQGACPQCHGLGRVYEVTEATMVPDPSLTIRERAVAAW
PMAWQGQNLRDILVTLGYDVDIPWRDLPQAQRDWILFTEETPTAPVYAGLTPAQTREALK
RKLEPSYQGTFMGARRYVLHTFMHSQSAQMRKRVAQYMHPSTCPLCQGKRLKREALAVTF
AGLDIAELSHLSLQALAEVFRKVAAADYLTQQQDELTLEKRLAAQRIAKELLERIDTLLD
LGLGYLALERSTPTLSSGELQRLRLATQLNSQLFGVVYVLDEPSAGLHPADSEALFEALQ
RLKQAGNSVYVVEHDLDTMRRADWLVDVGPAAGEHGGTILYSGPPAGLAEVQQSCTRTYL
FDAPVQATRAPRQARDWLKLEGITRNNLDNLSAAFPLGCFTAVTGISGSGKSSLVSQALL
ELVGAHLGHAEQRSEPEAQSLEDEPEQTSSGHVSAGLGSIKRLVQVDQKPIGRTPRSNLA
TYTGLFDHVRKCFAATEQAKAQGFDAGRFSFNVAKGRCANCEGEGFVSVELLFMPSVYAP
CPTCQGARYNPETLAVSWQGMNIAQVLQLTVDQALQVFAEQPPARRCLQVLQDIGLGYLR
LGQPATELSGGEAQRIKLATELQRMARGATLYVLDEPTNGLHPQDIDRLLVQLNRLVESG
HSVVVVEHDMRVVAQSDWVIDIGPGAGDAGGKVVVSGTPQVVAACTDSRTATFLAKAL