Protein Info for PP_2869 in Pseudomonas putida KT2440

Annotation: Oxidoreductase, FMN-binding

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 PF00724: Oxidored_FMN" amino acids 8 to 338 (331 residues), 197.6 bits, see alignment E=1.1e-61 PF07992: Pyr_redox_2" amino acids 388 to 621 (234 residues), 44.7 bits, see alignment E=3.7e-15 PF03486: HI0933_like" amino acids 388 to 427 (40 residues), 24.2 bits, see alignment 4e-09 PF13450: NAD_binding_8" amino acids 391 to 425 (35 residues), 28 bits, see alignment (E = 6.4e-10)

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2869)

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IY0 at UniProt or InterPro

Protein Sequence (650 amino acids)

>PP_2869 Oxidoreductase, FMN-binding (Pseudomonas putida KT2440)
MQTAFPLLFEPLQIRGKRLKNRIMSTGHDTCLPTDNLVNDKLIAYQRDRAAGGVGLIVLQ
VAGVHDSARYTSHVLMATDDACIDGYRQLAEACHAHGTVVLSQLFHPGREIMESADGLLA
VAYSASAVPNERFRVMPRALDQAMIDEIVQGYASAARRLHQAGLDGVEVVASHGYLPAQF
LNPRVNVRSDGYNGDLDQRLRFLREVLAAVRAATDEQFIIGLRISADERDSQGLSEDESL
AAAMALQGQLDYLHIVAGTSASLGGAVHIVPPMAIEPAYLAREAGTFKQQLAIPLFVTGR
INQPQEAELILARGQADVCGMTRALICDPSMPAKTERGQVEDVRACIACNQACIGHFHRG
LPISCIQRPETGRELQYGHLTPTSSPKRIMIAGGGPAGMKAAAVAAARGHQVTLYEAGPQ
LGGQVLLAQLLPRRSEFGGASTNLQREMALAGVEVVRNTRVDRALVERERPDLVIAATGA
TPYWPAFERTGELQVVDAWQLLRNEVKPGRSVLVIDWRCDWIGPGIAERLVREGHQVQLA
VNGTHCGESLPLYVRDQLAGELHRLGIPVTPYARLYGSDDNTVYLQHTASGDPMIFEGID
TLVLCMGHQPQDRLATELAGVVDVRRIGDCLAPRTAEEAIHDGLTVAWAL