Protein Info for PP_2844 in Pseudomonas putida KT2440

Annotation: Urease subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00192: urease, beta subunit
  • amino acids 1 to 102 (102 residues), 156 bits, E=1.4e-50
• PF00699: Urease_beta
  • amino acids 3 to 99 (97 residues), 153.6 bits, E=5.9e-50

Best Hits

Swiss-Prot: 100% identical to URE2_PSEPK: Urease subunit beta (ureB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01429, urease subunit beta [EC: 3.5.1.5] (inferred from 99% identity to ppf:Pput_2845)

MetaCyc: 70% identical to urease beta subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease beta subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

• urea degradation II (1/1 steps found)
• superpathway of allantoin degradation in plants (3/8 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Purine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

See Q88J05 at UniProt or InterPro

Protein Sequence (105 amino acids)

>PP_2844 Urease subunit beta (Pseudomonas putida KT2440)
MIPGEIQVAAGDIELNSGRETVSVSVANHGDRPVQVGSHYHFYEVNDALVFDRAPTLGFR
LDIPAGTAVRFEPGQARTVQLVAYAGKREVYGFQGKVMGALEGRA