Protein Info for PP_2818 in Pseudomonas putida KT2440

Annotation: Multidrug efflux RND transporter MexD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1042 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 341 to 360 (20 residues), see Phobius details amino acids 367 to 387 (21 residues), see Phobius details amino acids 394 to 414 (21 residues), see Phobius details amino acids 439 to 460 (22 residues), see Phobius details amino acids 473 to 498 (26 residues), see Phobius details amino acids 538 to 556 (19 residues), see Phobius details amino acids 868 to 887 (20 residues), see Phobius details amino acids 894 to 914 (21 residues), see Phobius details amino acids 921 to 943 (23 residues), see Phobius details amino acids 969 to 991 (23 residues), see Phobius details amino acids 1001 to 1026 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1023 (1023 residues), 1148.3 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1040 (1040 residues), 1401.5 bits, see alignment E=0 PF03176: MMPL" amino acids 343 to 497 (155 residues), 36.9 bits, see alignment E=2.2e-13

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 68% identity to axy:AXYL_03146)

Predicted SEED Role

"Multidrug efflux RND transporter MexD" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88J31 at UniProt or InterPro

Protein Sequence (1042 amino acids)

>PP_2818 Multidrug efflux RND transporter MexD (Pseudomonas putida KT2440)
MSRFFIHRPNFAWVVALFISLAGLLVIPSLPVAQYPNVAPPQISITASYPGASAKVMVES
VTSIIEQSLNGAKGLLYYESTNNSNGVAEVMVTFEPGTDPDMAQVDVQNRLKQAEARMPQ
AVLTQGLKVEQASSGFLLIYALTSTAGNRGDTTALADYAARNINNELLRVPGVGKLQFFA
SEAAMRVWVDPQKLVGYGLSIDDINSAIRGQNVQVPAGSFGSTPGASEQELTATLAVQGT
LDTPEAFAGIVLRANPDGSSVRLGDVARMAIGSENYNLSARLNGHPAVAGAVQLAPGANA
IQTATLVKERLAELSQFFPEGVEYSVPYDTSRFVDVAIEKVIHTLIEAMVLVFLVMFLFL
QNVRYTLVPSIVVPVCLLGTLMIMKLLGFSVNMMTMFGMVLAIGILVDDAIVVVENVERL
MAEEGLSPVEATIKAMGQVSGAIIGITLVLAAVFLPLAFMSGSVGVIYQQFSVSLAVSIL
FSGFLALTFTPALCATLLKPVPHGHHEKAGFFGAFNRGFARVTERYSLLNSELVARAGRW
MLAYVGILVVLGYSYLRLPEAFVPAEDLGYSVVDVQLPPGASRVRTDHTAEALEKFLMSR
DAVANSFIVSGFSFSGQGDNAALAFPTFKDWSQRDKAQSAEAETAAINAQFAANGDGAIT
AVMPPPIDGLGNSGGFALRLMDRGGLGREALLAARDQLLARANGNPVILYAMMEGLAEAP
QLRLHIDREKARALGVSFEAINSTLATAFGSAVINDFTNAGRQQRVVVQAEQGERMTPES
VLRLYAPNANGEQVPFSAFVTTQWEEGPVQLVRYNGYPSIRIAGDASPGHSTGQAMAEME
RLVSELPPGIGYAWTGLSYQEKVSSGQAASLFALAILVVFLLLVALYESWAIPLTVMLIV
PIGALGAVWAVTLTGMPNDVYFKVGLITIIGLAAKNAILIVEFAKELWEKGYSLCDAAIE
AARLRFRPIVMTSMAFILGVVPLAIASGAGAASQRAIGTGVIGGMLSATLLGVVFVPVCF
VWVLTLLKRKPSPVQQAVEEAE