Protein Info for PP_2805 in Pseudomonas putida KT2440

Annotation: FAD-containing monooxygenase EthA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details PF13738: Pyr_redox_3" amino acids 15 to 218 (204 residues), 35 bits, see alignment E=2e-12 PF13450: NAD_binding_8" amino acids 16 to 69 (54 residues), 29.7 bits, see alignment 1.3e-10 PF00743: FMO-like" amino acids 85 to 231 (147 residues), 47.5 bits, see alignment E=1.9e-16

Best Hits

Swiss-Prot: 100% identical to BVMO_PSEPK: Baeyer-Villiger monooxygenase (PP_2805) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2805)

MetaCyc: 100% identical to medium-chain acyclic ketone monooxygenase (Pseudomonas putida KT2440)
1.14.13.M76 [EC: 1.14.13.M76]; 1.14.13.M76 [EC: 1.14.13.M76]; 1.14.13.M76 [EC: 1.14.13.M76]; 1.14.13.M76 [EC: 1.14.13.M76]

Predicted SEED Role

"monooxygenase, flavin-binding family"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.M76

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88J44 at UniProt or InterPro

Protein Sequence (508 amino acids)

>PP_2805 FAD-containing monooxygenase EthA (Pseudomonas putida KT2440)
MSSHTALPVEPLDVLIMGAGVSGIGAAAYLRRNQPNKTFAILESRERMGGTWDLFRYPGI
RSDSDLYTFGFDFKPWTKAKSLADAADILEYLSEAIDEHQLAPFIQYQQKVISANWQSDK
GLWSVRVEDGRTAQIRTVECRWLFSAGGYYRYDQGFSPRFEGSEQFKGQIIHPQHWPEDL
DYTGKRVVVIGSGATAVTLIPAMADKVASITMLQRTPSYIINQPANDGVAAFLRKVLPAQ
TAYSLTRYKNAKITLAFWGFCQRFPKLSKKLLLWLTRKELPKDYPVDVHFNPPYNPWDQR
LCSVPEGDLFKAISAGNADIVTDHIERFTEHGVLLKSGKMLKADIIVTATGLNVQLFGGI
TLHKDGKPVVLSETLAYKGMMLSGVPNFAFAVGYTNSSWTLKVCLLCDHFCRLLGLMERE
GYNVCEPKAPEGVETRPLLDFGAGYVQRALDSMPRQGPREPWVMSMDYFRDVKLLRRGAV
TDKCLKFTAVPNAPLHADVQLQQQGSRR