Protein Info for PP_2791 in Pseudomonas putida KT2440

Updated annotation (from data): 4-hydroxypentanoyl-CoA kinase, subunit LvaA
Rationale: Specific phenotype: utilization of 4-Hydroxyvalerate, Levulinic Acid. See PMID:28947739
Original annotation: Aminoglycoside phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 PF01636: APH" amino acids 66 to 310 (245 residues), 139.6 bits, see alignment E=7.7e-45

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2791)

MetaCyc: 100% identical to LvaA (Pseudomonas putida KT2440)
2.7.1.M16 [EC: 2.7.1.M16]

Predicted SEED Role

"aminoglycoside phosphotransferase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.M16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88J58 at UniProt or InterPro

Protein Sequence (376 amino acids)

>PP_2791 4-hydroxypentanoyl-CoA kinase, subunit LvaA (Pseudomonas putida KT2440)
MKSWPRPCSWACANPETKGRAYRTMSSSPTISPASDTFAAMTDDHRLAEFIREQASATRV
VIQARKRLSGGAIQENWLLDLLIEGGPWAGVRRWVLRSDALSALPASLDREQEFAVLQVV
YQAGVKVPRPLWLCRDVRVHGRVFFLMEYVPGSAAGRALSTGAGPQGRAQLATQLGANLA
RLHQVRPPCATLCFLSVPDSSPALATIDAYRRYLDTLADAYPVLEWGLRWCELHAPRSST
LCLLHRDYRTGNYLASEEGLEAVLDWEFTGWGDPCEDLGWFTARCWRFTRPDLEAGGIGQ
LEDFLRGYHEVSSLCIERSRLHYWQVMATLRWAVIALQQGQRHLSGEEPSLELALTARLL
PELELDILHMTGAEAP