Protein Info for PP_2741 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 545 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 147 to 166 (20 residues), see Phobius details amino acids 213 to 235 (23 residues), see Phobius details amino acids 253 to 281 (29 residues), see Phobius details amino acids 309 to 328 (20 residues), see Phobius details amino acids 340 to 365 (26 residues), see Phobius details PF00924: MS_channel_2nd" amino acids 359 to 420 (62 residues), 60.8 bits, see alignment E=1.1e-20 PF21082: MS_channel_3rd" amino acids 429 to 514 (86 residues), 32.2 bits, see alignment E=1.2e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2741)

Predicted SEED Role

"FIG00963008: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JA8 at UniProt or InterPro

Protein Sequence (545 amino acids)

>PP_2741 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MGMKRRLGRAAWLMLLIHLLFSPLLHAGTPQAGPEIEGVIAEPAPLTVINRTVLVFRATL
LGETPKVRAQRAKKVIEETLQETNDLEVRVDPILRSYLVLLGGRRAFIVTPLDAAADNIT
TGEAAEQAAENLRLVVEETRQARSLRFLLTAIGYSAAATLVFFMLVKAAGYCRRKLLGLL
PKLMGKHSERLKVGHTQLFDLQNLYYLIDRSLWLLYWLVVLLFCYQWLGFVLSQFPYTRS
WGESLNIHLMALLNYLISGILRAMPGLAVALSIFFIARGISAFSKRVLRRMARPGTLKWL
TAETLQPTMRLTSLAIWLFALVMAYPYLPGSGTDAFKGLSVLLGLMISLGATSVIGQGAA
GLILTYTRTLRAGEFVRVGDHEGTVTEVGMFTTTIRTGLGEVLTLPNSMITGSVTKNYSR
IVQGPGYVVDTVVTIGYDTPWRQVEAMLLEAARRTDGVLQTPTPQVFQTALSDFYPEYRL
VAQAIPSEPRPRAQLLSLLHANIQDVFNEYGVQIMSPHYLGDPEQPKWVPKEQWHAAPSK
PENTG