Protein Info for PP_2528 in Pseudomonas putida KT2440

Annotation: O-acetylhomoserine (thiol)-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 2 to 421 (420 residues), 693.5 bits, E=4.3e-213
• PF01053: Cys_Met_Meta_PP
  • amino acids 4 to 421 (418 residues), 465.4 bits, E=2.2e-143
• PF01041: DegT_DnrJ_EryC1
  • amino acids 56 to 183 (128 residues), 32.8 bits, E=9e-12
• PF00155: Aminotran_1_2
  • amino acids 60 to 220 (161 residues), 34.9 bits, E=2.1e-12
• PF00266: Aminotran_5
  • amino acids 132 to 213 (82 residues), 25.5 bits, E=1.3e-09

Best Hits

Swiss-Prot: 54% identical to CYSD_SCHPO: Homocysteine synthase (met17) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 100% identity to ppf:Pput_3191)

MetaCyc: 53% identical to O-acetylhomoserine aminocarboxypropyltransferase (Rhodospirillum rubrum)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See Q88JW9 at UniProt or InterPro

Protein Sequence (425 amino acids)

>PP_2528 O-acetylhomoserine (thiol)-lyase (Pseudomonas putida KT2440)
MKLETLAIHAGFSPDPTTKAVAVPIYQTTSFAFDDTQHGADLFDLKVAGNIYSRIMNPTN
DVLEQRMAALEGGVGALAVASGMAAITYAIQTVAEAGDNIVSVAKLYGGTYNLLAHTLPR
MGIHTRFAAHDDIAALEALIDARTKAVFCESIGNPAGNIVDIAALAEAAHRHGVPLIVDN
TVATPVLCRPFEHGADIVVHSLTKYIGGHGTSIGGIVIDSGKFPWAENKERFALLNTPDP
SYHGVTYTEAFGPAAFIGRCRVVPLRNTGAALSPFNAFLILQGLETLALRMERHTENALK
VAHYLQAHEQVAWVKFAGLPDHPEHALAQRYTGGKPASILSFGIKGGQAAGARFIDALQL
VVRLVNIGDAKSLACHPASTTHRQLNDDELEKAGVPRDMVRLSIGIEHSDDIIADLAQAL
EASRG