Protein Info for PP_2488 in Pseudomonas putida KT2440

Annotation: NAD+-dependent succinate semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 3 to 453 (451 residues), 447.8 bits, E=1.9e-138

Best Hits

Swiss-Prot: 50% identical to GABD_DEIRA: Succinate-semialdehyde dehydrogenase [NADP(+)] (ssdA) from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 100% identity to ppu:PP_2488)

MetaCyc: 43% identical to succinate-semialdehyde dehydrogenase monomer (Synechococcus sp. PCC 7002)
SUCCSEMIALDDEHYDROG-RXN [EC: 1.2.1.79]

Predicted SEED Role

"Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16)" (EC 1.2.1.16, EC 1.2.1.24)

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• superpathway of 4-aminobutanoate degradation (3/3 steps found)
• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation II (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• GABA shunt II (3/4 steps found)
• 4-aminobutanoate degradation IV (2/3 steps found)
• GABA shunt I (2/4 steps found)
• N-methylpyrrolidone degradation (1/3 steps found)
• nicotine degradation II (pyrrolidine pathway) (6/11 steps found)
• 4-hydroxyphenylacetate degradation (2/8 steps found)
• nicotine degradation I (pyridine pathway) (5/17 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glutamate metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.24

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.24 or 1.2.1.79

Sequence Analysis Tools

See Q88K05 at UniProt or InterPro

Protein Sequence (463 amino acids)

>PP_2488 NAD+-dependent succinate semialdehyde dehydrogenase (Pseudomonas putida KT2440)
MAYATINPYNGELLKTFPETTDDQVNQALDDAHSAFLDWKERSFTERSAILQRAAELLRA
QASDYAHLLTLEMGKITAEALGEVEISARILEYYAKHAEGLLAPEKLPVAEPSEGEATLT
HEPLGIILAIEPWNFPYYQVARILAPQLAAGNTLLLKHASNVPQCAAAFEQLMLDAGLPS
GAFRNLYATRQQIESIISDDRVHGVALTGSEGAGAIIAAQAGKALKKSTLELGGADAFVV
LRDAELDKTVKWAVIGRHWNAGQVCISSKRMIVVDEVYEEFLSRYTEGVAQLKAGDPFAQ
ETTLAPLSSQAAADEIRQKIQVAVAHGARATEVGPRVPRLGAFVQPTILTDVEPDNPAYY
WEFFGPVSMIFRARDEQDAVRIANDSPFGLGGSVFTADTKHGAEVAKRISTGMVFVNHPS
TSRANLPFGGIKRSGYGRELIGLGIKEFVNHKLISVIDIDAPF