Protein Info for PP_2470 in Pseudomonas putida KT2440

Annotation: Phenylalanine--tRNA ligase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 793 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 792 (792 residues), 849.4 bits, see alignment E=1.5e-259 PF01588: tRNA_bind" amino acids 45 to 144 (100 residues), 78.5 bits, see alignment E=8.7e-26 PF03483: B3_4" amino acids 211 to 385 (175 residues), 240.7 bits, see alignment E=1.9e-75 PF03484: B5" amino acids 406 to 472 (67 residues), 75.7 bits, see alignment E=6.8e-25 PF17759: tRNA_synthFbeta" amino acids 475 to 683 (209 residues), 211.1 bits, see alignment E=3.4e-66 PF03147: FDX-ACB" amino acids 699 to 792 (94 residues), 95.9 bits, see alignment E=4.1e-31

Best Hits

Swiss-Prot: 100% identical to SYFB_PSEPK: Phenylalanine--tRNA ligase beta subunit (pheT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 100% identity to ppu:PP_2470)

MetaCyc: 53% identical to phenylalanine--tRNA ligase subunit beta (Escherichia coli K-12 substr. MG1655)
Phenylalanine--tRNA ligase. [EC: 6.1.1.20]; 6.1.1.20 [EC: 6.1.1.20]; 3.1.1.- [EC: 6.1.1.20]

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88K22 at UniProt or InterPro

Protein Sequence (793 amino acids)

>PP_2470 Phenylalanine--tRNA ligase beta subunit (Pseudomonas putida KT2440)
MKFSEQWLRGWVNPQVSRDELVARLSMAGLEVDSVTPAAGQFSGIVVGEILATEQHPDAD
KLRVCQVSSGQETFQVVCGAPNARPGIKIPFAMIGAELPGDFKIKKAKLRGVESFGMLCS
AAELQISEENDGLLELAADAPVGEDIRTYLSLDDASIEIGLTPNRGDCLSIAGLARDVSA
LYDTPVTRPVVPAVAAAHDEVRPVEVSAPAACPRYLGRVIRNVDLSKPTPLWMVERLRRS
DVRSIDAAVDITNYVMLELGQPMHAFDLAEINGGIRVRMAEEGEKLVLLDGQEVALRADT
LVIADHTRALAIAGVMGGEHSGVNTEKTRDLFLESAFFEPISVAGKARSYGLHTDASHRY
ERGVDSQLAREAMERATQLLLDIVGGEAGPVVEAVSEQHLPQVAPVTLRAERITQMLGME
MDPAQVEQLLNALELTTTKSGEGQWTVSVPSHRFDISLEVDLIEELARLYGYNNLPVRYP
QARLAPQGKPETRGDLPTLRRLLVARGYQEAITYSFIDPKLFELFSPGVEPLLLANPISS
DMAAMRASLWPGLVKALQHNLNRQQDRVRLFESGLRFVGQLGDLQQQPMIAGVITGSRLP
EGWANGRDGVDFFDVKADVEALLGYSGALSDFTFSAGKHPALHPGQTAVIERDGKLVGYL
GAIHPELAKALGLDRPVFLFELVLGDVVEGRLPKFSELSKFPETRRDLALIAGRDVASSA
VLELIRDNAGEWLTDLRLFDVYQGKGIDPDRKSLAVGLTWQHPSRTLNDDEVNTTLQNIL
TSLEQRLNTTLRK