Protein Info for PP_2448 in Pseudomonas putida KT2440

Updated annotation (from data): Gamma-glutamylputrescine oxidase (EC 1.4.3.-)
Rationale: Specific phenotype: utilization of Putrescine Dihydrochloride
Original annotation: gamma-glutamylputrescine oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01946: Thi4
  • amino acids 28 to 77 (50 residues), 27.6 bits, 2.5e-10
• PF01494: FAD_binding_3
  • amino acids 29 to 62 (34 residues), 25.2 bits, 1.4e-09
• PF01266: DAO
  • amino acids 31 to 381 (351 residues), 229.6 bits, E=1.3e-71

Best Hits

Swiss-Prot: 65% identical to PUUB_ECOLI: Gamma-glutamylputrescine oxidoreductase (puuB) from Escherichia coli (strain K12)

KEGG orthology group: K09471, gamma-glutamylputrescine oxidase [EC: 1.4.3.-] (inferred from 100% identity to ppg:PputGB1_3524)

MetaCyc: 65% identical to gamma-glutamylputrescine oxidase (Escherichia coli K-12 substr. MG1655)
1.4.3.M3 [EC: 1.4.3.M3]

Predicted SEED Role

"Nucleoside-diphosphate-sugar epimerases"

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• superpathway of ornithine degradation (6/8 steps found)
• putrescine degradation II (3/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.- or 1.4.3.M3

Sequence Analysis Tools

See Q88K44 at UniProt or InterPro

Protein Sequence (427 amino acids)

>PP_2448 Gamma-glutamylputrescine oxidase (EC 1.4.3.-) (Pseudomonas putida KT2440)
MANTPYPQSYYAASANPVPPRPALQGEVETDVCIIGAGYTGLSSALFLLENGFKVSIVEA
AKVGFGASGRNGGQIVNSYSRDIDVIERTVGPKQAQLLGHMAFEGGRIIRERVAKYNIQC
DLKDGGVFAALTSKQMGHLESQKRLWERFGHNQLELMDQKRIREVVACDSYIGGMLDMSG
GHIHPLNLALGEAAAVESLGGIIYEQTPAVRIERGANPVVHTPQGKVRAKFIIVAGNAYL
GNLVPELAAKSMPCGTQVITTEPLGEELARTLLPQDYCVEDCNYLLDYYRLTSDKRLIFG
GGVVYGARDPANIEAIIRPKMLKAFPQLKNVKIDYAWTGNFLLTLSRLPQVGRIGDNIYY
SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFAGLPHYPFPGGQMLRVPFSAIGAWYYS
LRDRLGF