Protein Info for PP_2437 in Pseudomonas putida KT2440

Annotation: putative Acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 PF02771: Acyl-CoA_dh_N" amino acids 13 to 133 (121 residues), 51.4 bits, see alignment E=2.8e-17 PF02770: Acyl-CoA_dh_M" amino acids 137 to 238 (102 residues), 76.7 bits, see alignment E=2.6e-25 PF00441: Acyl-CoA_dh_1" amino acids 250 to 398 (149 residues), 117 bits, see alignment E=1.8e-37 PF08028: Acyl-CoA_dh_2" amino acids 267 to 372 (106 residues), 41.5 bits, see alignment E=3.2e-14

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 100% identity to ppu:PP_2437)

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)" (EC 1.3.8.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4, 1.3.99.3

Use Curated BLAST to search for 1.3.8.4 or 1.3.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88K54 at UniProt or InterPro

Protein Sequence (414 amino acids)

>PP_2437 putative Acyl-CoA dehydrogenase (Pseudomonas putida KT2440)
MKVAQMDFAYSPKVQALRERVTAFMDAHVYPAEAVFERQVAEGDRWQPTAIMEELKAKAR
AEGLWNLFLPESEYGAGLSNLEYAPLAEIMGRSLLGPEPFNCSAPDTGNMEVLVRYGSEA
QKRQWLEPLLRGEIRSAFAMTEPDVASSDATNMAATAIRDGDQWVINGRKWWTSGACDPR
CKVMIFMGLSDPEGPRHQQHSMVLVPTDTPGVKIVRPLPVFGYDDAPHGHAEVLFENVRV
PYENVILGEGRGFEIAQGRLGPGRIHHCMRSIGMAERALELMCKRSVERTAFGRPLARLG
GNVDKIADSRMEIDMARLLTLKAAYMMDTVGNKVARSEIAQIKVVAPNVALNVIDRAIQI
HGGAGVSGDFPLAYMYAMQRTLRLADGPDEVHRAAIGKYEIGKYVPVEMLRSGR