Protein Info for PP_2338 in Pseudomonas putida KT2440

Annotation: 2-methylcitrate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 TIGR02330: 2-methylcitrate dehydratase" amino acids 14 to 494 (481 residues), 866.9 bits, see alignment E=1.7e-265 PF03972: MmgE_PrpD_N" amino acids 18 to 268 (251 residues), 309.2 bits, see alignment E=1.7e-96 PF19305: MmgE_PrpD_C" amino acids 300 to 476 (177 residues), 198.9 bits, see alignment E=5.9e-63

Best Hits

Swiss-Prot: 61% identical to MMGE_BACSU: Citrate/2-methylcitrate dehydratase (mmgE) from Bacillus subtilis (strain 168)

KEGG orthology group: K01720, 2-methylcitrate dehydratase [EC: 4.2.1.79] (inferred from 100% identity to ppu:PP_2338)

Predicted SEED Role

"2-methylcitrate dehydratase (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.79

Use Curated BLAST to search for 4.2.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KF2 at UniProt or InterPro

Protein Sequence (494 amino acids)

>PP_2338 2-methylcitrate dehydratase (Pseudomonas putida KT2440)
MSANVDLNDRPDYDRVLQTLADYALGYRVDSREALDTARNCLMDTLGCGLLALRFPECTK
LLGPQVEGTLVPNGARVPGTSYRLDPVKAAWDIGCTVRWLDYNDTWLAAEWAHPSDNLGG
ILAVADHLSQKRVAAGEKPLLMRDVLEAMVMAHEIQGVLALENAFNRVGLDHVILVKVAS
TAVCARLMGANREQMLSALSHAFVDGQALRTYRHAPNAGSRKSWAAGDASSRGVRLADIA
LRGEMGVPGVLTASQWGFYDVSFSHTNKDLALKPAGQYELRLPQALGSYVMENVLFKVSF
PAEFHAQTACEAAVTLHPLVRNRLHEVDRIVITTQESAIRIISKSGPLANAADRDHCLQY
MVAVPLIFGHLVAEHYEDAFHANHPSIDRLREKMEVVEDPRFSREYLEPGKRSIANALQV
FFKDGSSTEQVVVEYPIGHRRRRGEGIPLLEAKFRANLATRFARQRCQQVFELCKDQQKL
EGTAVHRFVDLFVI