Protein Info for PP_2329 in Pseudomonas putida KT2440

Annotation: aminodeoxychorismate synthase / para-aminobenzoate synthase multi-enzyme complex

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04715: Anth_synt_I_N
  • amino acids 14 to 135 (122 residues), 67.9 bits, E=1.2e-22
• TIGR00553: aminodeoxychorismate synthase, component I
  • amino acids 93 to 439 (347 residues), 355.4 bits, E=1.4e-110
• PF00425: Chorismate_bind
  • amino acids 181 to 435 (255 residues), 311.6 bits, E=4.6e-97

Best Hits

KEGG orthology group: K01665, para-aminobenzoate synthetase component I [EC: 2.6.1.85] (inferred from 100% identity to ppf:Pput_3441)

Predicted SEED Role

"Para-aminobenzoate synthase, aminase component (EC 2.6.1.85)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. or Folate Biosynthesis (EC 2.6.1.85)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• superpathway of candicidin biosynthesis (4/11 steps found)
• chloramphenicol biosynthesis (1/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.85

Use Curated BLAST to search for 2.6.1.85

Sequence Analysis Tools

See Q88KG1 at UniProt or InterPro

Protein Sequence (446 amino acids)

>PP_2329 aminodeoxychorismate synthase / para-aminobenzoate synthase multi-enzyme complex (Pseudomonas putida KT2440)
MPTCTLHPLPYQPDPAAYFAHLRQAPGAILLDSARPGAERGRFDLLSAWPLQQLQAQPDE
DGRVFLQRLRAGLAQLGHADLPNGVELPFAGGLIGYLSYDFGRRLEHLPSLAVDDLGLPD
AQLGLYAWALVSDHLLGTSQLVFHPSLAGDERERLISLFQDTSATTGDFRLLAPMAGDLE
PEQYKTAFDQVQRYIQAGDCYQINLTQRFRAPCQGDPWRAYQALRQACPTPFSGYQQLAD
GSTLLSFSPERFIRVSQGQVETRPIKGTRPRASDPAQDQRNAQELLHSPKDRSENLMIVD
LLRNDLGRTCEIGSVKVPELFSLESYPNVHHLVSSITGQLASDKDALDLIGDSFPGGSIT
GAPKIRAMQIIDELEPARRALYCGSLLYVDVRGEMDSSIAIRSLLIKDGQVSCWGGGAVV
ADSQWQAEYEESIAKVRVLMQTLQGL