Protein Info for PP_2327 in Pseudomonas putida KT2440

Annotation: transcriptional dual regulator / CysB-O-acetyl-L-serine

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 324 PF00126: HTH_1" amino acids 3 to 64 (62 residues), 55.7 bits, see alignment E=4e-19 PF03466: LysR_substrate" amino acids 89 to 269 (181 residues), 140.5 bits, see alignment E=5.2e-45

Best Hits

Swiss-Prot: 65% identical to CYSB_SALTY: HTH-type transcriptional regulator CysB (cysB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K13634, LysR family transcriptional regulator, cys regulon transcriptional activator (inferred from 100% identity to ppf:Pput_3443)

MetaCyc: 64% identical to DNA-binding transcriptional dual regulator CysB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Cys regulon transcriptional activator CysB" in subsystem Cysteine Biosynthesis or DNA-binding regulatory proteins, strays

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KG3 at UniProt or InterPro

Protein Sequence (324 amino acids)

>PP_2327 transcriptional dual regulator / CysB-O-acetyl-L-serine (Pseudomonas putida KT2440)
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVT
PAGERIINTAGEILRKVESIKQIAQEFSNEKKGTLSIATTHTQARYALPPVISSFIKQYP
EVSLHMHQGSPMQIAEMAADGTVDFAIATEALELFGDLIMMPCYKWNRCVVVPQGHPLAK
LPKLTLEAVAEYPIVTYVFGFTGRSKLDEAFNHRGLTPKVVFTAADADVIKTYVRLGLGV
GIVAGMAVDAKLDSDLVALDASELFEASITKIGFRRGTFLRGFMCDFIEKFAPHLTREVM
AKAIQCHNKQELEELFEGVELPVH