Protein Info for PP_2302 in Pseudomonas putida KT2440

Annotation: DNA-binding, ATP-dependent protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 PF02190: LON_substr_bdg" amino acids 7 to 197 (191 residues), 198.1 bits, see alignment E=4.9e-62 TIGR00763: endopeptidase La" amino acids 8 to 766 (759 residues), 996.4 bits, see alignment E=6.1e-304 PF08298: AAA_PrkA" amino acids 316 to 369 (54 residues), 25.1 bits, see alignment 2.7e-09 PF07728: AAA_5" amino acids 348 to 481 (134 residues), 39.6 bits, see alignment E=1.6e-13 PF00004: AAA" amino acids 348 to 485 (138 residues), 80.8 bits, see alignment E=3.8e-26 PF05362: Lon_C" amino acids 564 to 767 (204 residues), 325.4 bits, see alignment E=4.1e-101 PF13541: ChlI" amino acids 655 to 735 (81 residues), 27.8 bits, see alignment E=6e-10

Best Hits

Swiss-Prot: 70% identical to LON_ECOL6: Lon protease (lon) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 100% identity to ppu:PP_2302)

MetaCyc: 70% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KI8 at UniProt or InterPro

Protein Sequence (798 amino acids)

>PP_2302 DNA-binding, ATP-dependent protease (Pseudomonas putida KT2440)
MKTTLDLPLLPLRDVVVYPHMVIPLFVGREKSIEALEAAMTGEKQILLLAQKNPADDDPG
EDALYRVGTVATVLQLLKLPDGTVKVLVEGEQRGAVERFSEVEGHIRAEVSLIDETDTAE
RESEVFVRTLLSQFEQYVQLGKKVPAEVLSSLNSIDEPGRLVDTMAAHMALKIEQKQEIL
EIVDLTTRVEHVLALLDAEIDLLQVEKRIRGRVKKQMERSQREYYLNEQMKAIQKELGDG
DEGHNEVEELKKRIEAAGLPKDALAKAQAELNKLKQMSPMSAEATVVRSYLDWLVQVPWK
AQSKVRLDLAKAEEILDADHYGLEEVKERILEYLAVQKRVKKIRGPVLCLVGPPGVGKTS
LAESIAAATNRKFVRMALGGVRDEAEIRGHRRTYIGSMPGRLIQKMTKVGVRNPLFLLDE
IDKMGSDMRGDPASALLEVLDPEQNHNFNDHYLEVDYDLSDVMFLCTSNSMNIPPALLDR
MEVIRLPGYTEDEKINIAVKYLVPKQVKANGLKKEELEVDVSAIRDIIRYYTREAGVRGL
ERQIAKVCRKVVKEHTGQKQVKVKVASEQLEHLLGVRKFRYGLAEQQDQIGQVTGLAWTQ
VGGELLTIEAVVIPGKGQLIKTGSLGDVMVESITAAQTVVRSRARSLGIAADFHEKHDVH
IHMPEGATPKDGPSAGIGMCTALVSALTQIPVRADVAMTGEITLRGQVLAIGGLKEKLLA
AHRGGIKTVIIPEENVRDLKEIPENIKQDLQIKPVKWIDEVLQIALQYAPEPLPDVAPEI
VAKDEKRDGDAKERISTH