Protein Info for PP_2301 in Pseudomonas putida KT2440

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 27 to 428 (402 residues), 690.2 bits, see alignment E=5.2e-212 PF06689: zf-C4_ClpX" amino acids 30 to 66 (37 residues), 70.6 bits, see alignment 3.2e-23 PF00493: MCM" amino acids 85 to 210 (126 residues), 23.8 bits, see alignment E=9.1e-09 PF01078: Mg_chelatase" amino acids 128 to 154 (27 residues), 21.1 bits, see alignment (E = 7.3e-08) PF07724: AAA_2" amino acids 129 to 326 (198 residues), 121.5 bits, see alignment E=1.6e-38 PF07728: AAA_5" amino acids 131 to 208 (78 residues), 26.2 bits, see alignment E=2.8e-09 PF00004: AAA" amino acids 132 to 238 (107 residues), 57.2 bits, see alignment E=1e-18 PF10431: ClpB_D2-small" amino acids 333 to 411 (79 residues), 43.6 bits, see alignment E=9.9e-15

Best Hits

Swiss-Prot: 100% identical to CLPX_PSEPK: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 100% identity to ppu:PP_2301)

MetaCyc: 77% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KI9 at UniProt or InterPro

Protein Sequence (442 amino acids)

>PP_2301 ATP-dependent Clp protease ATP-binding subunit ClpX (Pseudomonas putida KT2440)
MPSSCVASLPDWIDRMTDTRNGEDSGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVDLC
NDIIREEVQEAQAESSAHKLPSPKEISGILDQYVIGQERAKKVLAVAVYNHYKRLNQRDK
KGDEVELGKSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVENIIQ
KLLQKCDYDVEKAQMGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVASVP
PQGGRKHPQQEFLQVDTRNILFICGGAFSGLEKVIQNRSTKGGIGFGAEVRSKEEGKKVG
ESLREVEPDDLVKFGLIPEFVGRLPVLATLDELDEAALMQILTEPKNALTKQYAKLFEME
SVDLEFRSDALKAVARKALERKTGARGLRSILEGVLLDTMYEIPSKKDVSKVVIDESVIE
GTSQPLMIYENSEPQAKAAPDA