Protein Info for PP_2264 in Pseudomonas putida KT2440

Annotation: putative Sugar ABC transporter, periplasmic sugar-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 579 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF13416: SBP_bac_8" amino acids 89 to 441 (353 residues), 34.8 bits, see alignment E=8e-13

Best Hits

KEGG orthology group: K02027, multiple sugar transport system substrate-binding protein (inferred from 100% identity to ppf:Pput_3473)

Predicted SEED Role

"Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization (TC 3.A.1.1.3)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KM6 at UniProt or InterPro

Protein Sequence (579 amino acids)

>PP_2264 putative Sugar ABC transporter, periplasmic sugar-binding protein (Pseudomonas putida KT2440)
MFDNKHNRRHLTLAALLVLASVQGTAWADQYEEAAKKWIGSEFKPSTLTPEQQLAELQWF
IKAAEPFRGMKINVVSETITTHEYESKVLAKAFSEITGIQLTHDLLQEGDVVEKLQTQMQ
SDKNIYDGWVNDSDLIGTHFRYGKVESITDLMANEGKAYTSPTLDLKDFIGISFTTAPDG
KVYQLPDQQFANLYWFRADWFERPELKARFKEKYGYELGVPVNWSAYEDIAKFFTEDVKE
IDGKRVYGHMDYGKKDPSLGWRFTDAWFSMAGGGDKGLPNGLPVDEWGIRVEDCHPVGSS
VTRGGDTNGPAAVYATQKYVDWMRAYAPKEAQGMTFSEAGPVPAQGNIAQQIFWYTAFTA
DMTKPGLPVVNADGTPKWRMAPSPKGPYWEEGMKLGYQDTGSWTFFKSTPEKQRLAAWLY
AQFVTSKTVSLKKTIVGLTPIRESDINSQAMTELAPKLGGLVEFYRSPARVQWTPTGTNV
PDYPRLAQLWWSHIAEVASGEKTPQEALDGLARDQDRMMERLQRSNAQATCAPKLNPEKD
AQYWFDQPGAPKAKLANEKPMGETVSYNELLKSWEAARK