Protein Info for PP_2213 in Pseudomonas putida KT2440

Annotation: putative acyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 58 to 401 (344 residues), 232.8 bits, E=5.9e-73
• PF13193: AMP-binding_C
  • amino acids 460 to 537 (78 residues), 90.3 bits, E=1.2e-29

Best Hits

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to ppu:PP_2213)

Predicted SEED Role

"Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases"

MetaCyc Pathways

• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• chitin deacetylation (2/4 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1

Sequence Analysis Tools

See Q88KS6 at UniProt or InterPro

Protein Sequence (561 amino acids)

>PP_2213 putative acyl-CoA synthetase (Pseudomonas putida KT2440)
MLQLQKQETRSPMMRDYAEAARTFDHDQAVVTALHGNLEALNACVECCDRHAGDGKLALI
HEDRDGNSACYSFDQLQVQAARFANVLKAQGVGAGDRVAGLMPRTPELLVTILATWRLGA
VYQPLFTAFGPKAIEHRLEQSHARVVVTDSHNRAKLDDVHACPTIITVKARSGELDFQQC
LERAADVCEPVMRSGNDPFLLMFTSGTTGPAKPLEVPLRAIVAFQGYMRDAIELLPEDNF
WNLADPGWAYGLYYAVTGPLSLGHATTFYDGPFSVESCARVIDKLGITNLAGSPTAYRLL
IAAGKDFSAPIKGRLRVVSSAGEPLNPEVIRWFADELGVTIHDHYGQTELGMVLCNHHGL
QHPVHLGSAGYAIPGHRIVVVDEQGNELPAGQPGILAVDREQSPLCWFGGYHGLPTKAFV
GKYYLSGDTVELNPDGSISFVGRSDDVITTSGYRVGPFDVESALIEHPAVIEAAVIGKPD
PERTELIKAFVVLASGYAGSVELEETLRQHVRQRLYAHAYPREIEFVSELPKTPSGKLQR
FILRNQEVAKQQAQQATPASV