Protein Info for PP_2209 in Pseudomonas putida KT2440

Annotation: 2-aminoethylphosphonate--pyruvate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 TIGR02326: 2-aminoethylphosphonate--pyruvate transaminase" amino acids 3 to 361 (359 residues), 558.7 bits, see alignment E=5.6e-172 TIGR03301: 2-aminoethylphosphonate aminotransferase" amino acids 6 to 359 (354 residues), 524.1 bits, see alignment E=2.1e-161 PF00266: Aminotran_5" amino acids 36 to 301 (266 residues), 62.2 bits, see alignment E=2.3e-21

Best Hits

Swiss-Prot: 100% identical to PHNW_PSEPK: 2-aminoethylphosphonate--pyruvate transaminase (phnW) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03430, 2-aminoethylphosphonate-pyruvate transaminase [EC: 2.6.1.37] (inferred from 100% identity to ppu:PP_2209)

MetaCyc: 80% identical to 2-aminoethylphosphonate aminotransferase monomer (Pseudomonas aeruginosa)
2-aminoethylphosphonate--pyruvate transaminase. [EC: 2.6.1.37]

Predicted SEED Role

"2-aminoethylphosphonate:pyruvate aminotransferase (EC 2.6.1.37)" in subsystem Phosphonate metabolism (EC 2.6.1.37)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KT0 at UniProt or InterPro

Protein Sequence (368 amino acids)

>PP_2209 2-aminoethylphosphonate--pyruvate transaminase (Pseudomonas putida KT2440)
MSNAPILLTPGPLTTSIRTRQAMLVDWGSWDRDFNQLTASVCEQLLAIIDGASSHHCVPL
QGSGTFAVEAAIGTLVPRDGKVLVLINGAYGQRLAKICKVLGRTYSTFETAEDQPTTAAD
VDRLLAEDPAITHVALIHCETSTGILNPLPEIAQVIKRHGKRLIIDAMSSFGALPIDARE
IPFEALIAASGKCLEGVPGMGFVFAEKSALAAAEGNAHSLAMDLHDQHAYMAKTGQWRFT
PPTHVVAALHEALQQYNEEGGLPARHQRYADNCKTLLDGMAAIGLRSFLPAEIQAPIIVT
FHAPTDARYQFKDFYERVKAKGFILYPGKLTQVETFRVGCIGVVGADGMQAAVNAVAEVL
REMEVLDI