Protein Info for PP_2145 in Pseudomonas putida KT2440
Annotation: beta-N-acetylglucosaminidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to NAGZ_PSEAB: Beta-hexosaminidase (nagZ) from Pseudomonas aeruginosa (strain UCBPP-PA14)
KEGG orthology group: K01207, beta-N-acetylhexosaminidase [EC: 3.2.1.52] (inferred from 100% identity to ppu:PP_2145)MetaCyc: 84% identical to beta-N-acetylhexosaminidase (Pseudomonas aeruginosa PAO1)
Beta-N-acetylhexosaminidase. [EC: 3.2.1.52]
Predicted SEED Role
"Beta N-acetyl-glucosaminidase (EC 3.2.1.52)" (EC 3.2.1.52)
MetaCyc Pathways
- peptidoglycan recycling II (8/10 steps found)
- peptidoglycan recycling I (10/14 steps found)
- chitin degradation II (Vibrio) (2/6 steps found)
- chitin degradation III (Serratia) (2/7 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Glycosaminoglycan degradation
- Glycosphingolipid biosynthesis - ganglio series
- Glycosphingolipid biosynthesis - globo series
- Nucleotide sugars metabolism
- Other glycan degradation
Isozymes
No predicted isozymesUse Curated BLAST to search for 3.2.1.52
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88KZ4 at UniProt or InterPro
Protein Sequence (336 amino acids)
>PP_2145 beta-N-acetylglucosaminidase (Pseudomonas putida KT2440) MTVSLQGSLMVDIAGKWLTAEDRQLLRQPEVAGLIIFARNIDNPRQVRELCASIRAIRPD LILAVDQEGGRVQRLRQGFVRLPAMRALADNDNAEYLAEQCGWLMATEVLAVGLDLSFAP VLDLDHQRSAVVGSRAFEGDPLLATRLAGAFIRGMNAAGMAACGKHFPGHGWAEADSHVA IPTDERSLEQLRQSDLVPFTRLSGQLAAVMPAHVIYPQVDNQPAGFSRRWLQDILRGELG FDGVIFSDDLSMAGAHVVGDAASRIEAALSAGCDMGLVCNDRAAAELALSAAQRMKVKPS PRIARMRGQGFARTDYRQQPRWLEALGALKEAQLVD