Protein Info for PP_2123 in Pseudomonas putida KT2440

Annotation: molybdenum::molybdopterin ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 PF03453: MoeA_N" amino acids 14 to 175 (162 residues), 160.5 bits, see alignment E=4.3e-51 TIGR00177: molybdenum cofactor synthesis domain" amino acids 184 to 319 (136 residues), 108.7 bits, see alignment E=1.2e-35 PF00994: MoCF_biosynth" amino acids 188 to 323 (136 residues), 112.4 bits, see alignment E=2.2e-36 PF03454: MoeA_C" amino acids 339 to 403 (65 residues), 51.9 bits, see alignment E=1.1e-17

Best Hits

KEGG orthology group: K03750, molybdopterin biosynthesis protein MoeA (inferred from 100% identity to ppu:PP_2123)

Predicted SEED Role

"Molybdopterin biosynthesis protein MoeA" in subsystem Molybdenum cofactor biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L14 at UniProt or InterPro

Protein Sequence (408 amino acids)

>PP_2123 molybdenum::molybdopterin ligase (Pseudomonas putida KT2440)
MTAVAEVSQARPLMPVEQALEQLLALAEAAPIRETENLPLAEAEGRVLATDLVASLDLPP
WPNSAMDGYALRLADWQGQPLPVSQRIFAGHAPQPLQPGTCARIFTGAPVPEGADCVEMQ
ENTEVLEDGRVRFLEALRTEQNIRPQGQETRKGEQVMSAGTRLGPIELGLAATLGHGRLD
VVRKVKVAVLSTGDELVEPGLPLGPGQIYNSNRRLLVSWLQRLGCEVVDAGILPDDLART
RACLAELGGVDLILSTGGVSVGEADYLGAALREAGELALWKLAIKPGKPLTFGHFQGVPV
IGLPGNPASTLVTFGLLTRPYLLRRQGVIDVTPLRFDMPAGFDWPKPGTRREYLRARIEQ
GRVRIYKNQSSGVLRSAAWAEGLVEVREGSTPKQGDIVPFIPFSELLG