Protein Info for PP_2094 in Pseudomonas putida KT2440
Annotation: putative Nitrate-binding protein NasS
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 100% identity to ppf:Pput_3646)Predicted SEED Role
"Nitrate ABC transporter, nitrate-binding protein" in subsystem Nitrate and nitrite ammonification
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88L41 at UniProt or InterPro
Protein Sequence (404 amino acids)
>PP_2094 putative Nitrate-binding protein NasS (Pseudomonas putida KT2440) MNTVPRATPLAWVNGSDAPEKTSLNIGYMALTDCASVVVAATQGFAQQHGLTLNLQRQGS WAGLRDKLVSGELDAAHCLYGLVYAVHLGIGGVPASDMAVLMGLNQNAQAINLSPALQRK GVTNPEALARLVHQHGARLTFAQTFPTGTHAMWLYYWLASQGIHPLRDVDSVVVPPAQMA AHLQAGRIDGFCVGEPWAADAVAKGQGFTLATSQSIWPDHPEKVLACARVFAEQYPNSAR ALIKAILTASRFIEQSPENRRSTAQLLSASAYLDTPLDSIAPRLLGHYEDGLGNQWQDPH ALRLFDQGRANLPYLSDGMWFMTQFRRWGLLREDPDYLGVARQVQQLQLYSEAAQSLGIA CPGQPMRSSLLIDGSRWDGSDPFGYARSFSLHALGELPDARVGA