Protein Info for PP_2082 in Pseudomonas putida KT2440

Annotation: phosphoenolpyruvate synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 5 to 786 (782 residues), 1215.2 bits, E=0
• PF01326: PPDK_N
  • amino acids 17 to 345 (329 residues), 428.9 bits, E=2.2e-132
• PF00391: PEP-utilizers
  • amino acids 382 to 454 (73 residues), 98.6 bits, E=2e-32
• PF02896: PEP-utilizers_C
  • amino acids 480 to 780 (301 residues), 211.6 bits, E=2.3e-66

Best Hits

Swiss-Prot: 94% identical to PPSA_PSEAB: Phosphoenolpyruvate synthase (ppsA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 89% identity to avn:Avin_23280)

MetaCyc: 68% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of anaerobic sucrose degradation (13/19 steps found)
• superpathway of N-acetylneuraminate degradation (12/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See Q88L53 at UniProt or InterPro

Protein Sequence (791 amino acids)

>PP_2082 phosphoenolpyruvate synthetase (Pseudomonas putida KT2440)
MVEYVVSLDKLGVHDVEHVGGKNASLGEMISNLAGAGVSVPGGFATTAQAYRDFLEQSGL
NDRIHAALDALDVDDINALTKTGAQIRQWVMEAEFPPRLDSEIRTAFAEMAAGNDNMAVA
VRSSATAEDLPDASFAGQQETFLNIRGVDNVIRAAKEVFASLFNDRAIAYRVHQGFDHKL
VALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITGAYGLGETVVQGAVNPDEFYVHK
NTLQAGRPAILRRNLGSKAIKMIYGDEAKAGRSVKTVEVDRAERARFCLTDAEVSELAKQ
AMIIEQHYQRPMDIEWAKDGDDGKLYIVQARPETVKSRSSANVMERYLLKEKGTVLVEGR
AIGQRIGAGKVRVINDVSEMDKVQPGDVLVSDMTDPDWEPVMKRASAIVTNRGGRTCHAA
IIARELGIPAVVGCGNATQVLKDGQGVTVSCAEGDTGFIFEGELGFDIKQNSVDAMPELP
FKIMMNVGNPDRAFDFAQLPNAGVGLARLEFIINRMIGVHPKALLNYAGLPADLKDSVDK
RIAGYNDPVGFYVEKLVEGISTLAAAFYPKKVIVRLSDFKSNEYANLIGGKLYEPEEENP
MLGFRGASRYISESFRDCFELECRALKRVRNEMGLTNVEIMVPFVRTLGEASQVVDLLAE
NGLARGDNGLRVIMMCELPSNAILAEEFLEYFDGFSIGSNDLTQLTLGLDRDSGIIAHLF
DERNPAVKKLLANAIAACNKAGKYIGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLET
WFFLAEGQGAA