Protein Info for PP_2077 in Pseudomonas putida KT2440

Annotation: diaminopimelate decarboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01048: diaminopimelate decarboxylase
  • amino acids 8 to 407 (400 residues), 398.3 bits, E=1.5e-123
• PF02784: Orn_Arg_deC_N
  • amino acids 39 to 266 (228 residues), 181.4 bits, E=2e-57
• PF00278: Orn_DAP_Arg_deC
  • amino acids 163 to 365 (203 residues), 86.5 bits, E=1.2e-28

Best Hits

Swiss-Prot: 60% identical to DCDA_STRCO: Diaminopimelate decarboxylase (lysA) from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

KEGG orthology group: K01586, diaminopimelate decarboxylase [EC: 4.1.1.20] (inferred from 100% identity to ppu:PP_2077)

MetaCyc: 60% identical to diaminopimelate decarboxylase (Escherichia coli K-12 substr. MG1655)
Diaminopimelate decarboxylase. [EC: 4.1.1.20]

Predicted SEED Role

"Diaminopimelate decarboxylase (EC 4.1.1.20)" in subsystem Lysine Biosynthesis DAP Pathway (EC 4.1.1.20)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-lysine biosynthesis II (6/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.20

Use Curated BLAST to search for 4.1.1.20

Sequence Analysis Tools

See Q88L58 at UniProt or InterPro

Protein Sequence (410 amino acids)

>PP_2077 diaminopimelate decarboxylase (Pseudomonas putida KT2440)
MTAPFTLLAEAVRQHGSPLWAYDTRTIADRVEQLNVFDTVRFAQKANPNLHVLRLMRAHG
LVLDAVSLGEMERAFAAGASVDGDPAGVVLTCDALDRPTLERVVASKIEVNAGSIDMLHQ
LGERSPGHRVWIRINPGFGHGHSRKTNTGGENSKHGIWHEELDEALACIKAHGLHLVGVH
MHIGSGVDYQHLEQVARSMIELIGRLGVDIEAFSIGGGLSTPYRSTDKPVDLQRYAQTWA
VARKEIEAMLGHPVRMEIEPGRFLVAESGYLVAEVRAVKQVGRNTFVIIDAGFNDLMRPA
MYGAYHGMTLLDADGQAVDRPRQPTVVAGPLCESGDVFTQDDQALTPQDLPQAQVGDLLV
IHDAGAYGASMSSNYNSRPLLPEFLIENGALRMIRRRQTVQDLLSLEADF