Protein Info for PP_2069 in Pseudomonas putida KT2440

Annotation: putative Multidrug efflux MFS outer membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 21 to 471 (451 residues), 244.9 bits, see alignment E=8.2e-77 PF02321: OEP" amino acids 70 to 263 (194 residues), 63.4 bits, see alignment E=1.2e-21 amino acids 292 to 470 (179 residues), 80.4 bits, see alignment E=7.8e-27

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2069)

Predicted SEED Role

"Outer membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L66 at UniProt or InterPro

Protein Sequence (473 amino acids)

>PP_2069 putative Multidrug efflux MFS outer membrane protein (Pseudomonas putida KT2440)
MMPLLHSFPGPARLATALGMLAALAACGHQPTPAEPAVALQADPLANMPAGVSKQPLPER
WWALYQDPQLNRWVQQALAHNQGLAQAEANVQAMLAGIGEFDARRWPSTDMGVAATYGRS
ADDQTLAEATDSNAPSQWQFNPGLELAYQVDIWGQVRAAIQRARVQAEASAAALDLVRLQ
VVAQTTRAYIDQCTYAARLEAAKQALQTLDQSVQLSQRQRQAGVATALDSERLLGLREQV
RAQLPMLSARRQMALYELSMLSGQASVAEATTCVAIPTLSVPLPAGDGWHLLERRPDVRQ
AERELQAATLETDIVRADLYPKVSFGASLTSSDHHLANLGDSRAVMFGIGPLIRWEFPNL
KANRARVSKAEALQRAQVARYRGVALSALKDVRQALARYDGERQRLQALDAALAHSQHSF
ALAQGNYRAGTVDGLALLDSEREMISLRANHTEARGRLAQAQVNLFRALGGRW