Protein Info for PP_2067 in Pseudomonas putida KT2440

Annotation: putative EmrB/QacA family drug resistance transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 51 to 72 (22 residues), see Phobius details amino acids 80 to 99 (20 residues), see Phobius details amino acids 105 to 127 (23 residues), see Phobius details amino acids 138 to 156 (19 residues), see Phobius details amino acids 168 to 189 (22 residues), see Phobius details amino acids 201 to 220 (20 residues), see Phobius details amino acids 232 to 254 (23 residues), see Phobius details amino acids 267 to 291 (25 residues), see Phobius details amino acids 303 to 323 (21 residues), see Phobius details amino acids 335 to 355 (21 residues), see Phobius details amino acids 368 to 389 (22 residues), see Phobius details amino acids 400 to 420 (21 residues), see Phobius details amino acids 480 to 498 (19 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 19 to 493 (475 residues), 364.1 bits, see alignment E=5.6e-113 PF07690: MFS_1" amino acids 19 to 413 (395 residues), 157.7 bits, see alignment E=1.9e-50

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2067)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L68 at UniProt or InterPro

Protein Sequence (504 amino acids)

>PP_2067 putative EmrB/QacA family drug resistance transporter (Pseudomonas putida KT2440)
MRTEQPVSLRAWVAVLGGLFGCFMAGMNVHVTSAALPEIRGSLGASFEEGSWISTAYLVA
EIVMIPLTAWLVDVFSLRRVMWTGSLIFLIASVACSWAPNLEAMIVIRVIQGAAGAVLIP
LSFQLIITELPASKMAMGMALFSLANSVAQAAGPSIGGWLTDAYSWRWIFYLQLFPGIAL
LLAIAWSIEAKPMKLELLRKGDWLGIAAMVIGLGGLQIVLEEGGRLDWFGSPLIVGMSVV
AAIALVVFVVTQLFGQRAFINLRLLGHYNFGVASVAMFIFGAATFGLVFLVPNYLSQLQG
FSAHDVGVALIAYGVVQLLLAPLMPRLMGWTSAKFMVASGFLIMALGCWLGAGLSADSAD
NVIIPSTVVRGIGQPFIMVALSVLAVAGLDKREAGSASAVFSMLRNLGGAIGTAGLTQLV
ATRERFHSERIHEQVTLFEPSVQARINQTVQSWLPPQQQMLEALEATIRREAYLMAYSDA
FYLACLALVGCALAALLLRARQQH